The role of host factors in LINE retrotransposition

宿主因子在 LINE 逆转录转座中的作用

• 批准号: 23770004
• 负责人: KAJIKAWA Masaki
• 金额: $ 2.91万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770004/
• 关键词: ゲノム構築; 再編; 維持; 転移因子; レトロトランスポゾン

Transposable elements, including LINEs, are major components of eukaryotic genomes. They mobilize and amplify their own copies via the reverse transcription of their own RNA. This mobilization mechanism is called retrotransposition, and it requires proteins encoded by transposable elements. However, retrotransposition is not completed by the proteins only, and host encoded proteins are most likely required for retrotransposition. This study found that a retrotransposition intermediate of LINEs contains a large amount of host encoded proteins, which probably participate in retrotransposition.

转座因子，包括LINE，是真核生物基因组的主要成分。它们通过自身RNA的逆转录来调动和扩增自身的拷贝。这种动员机制称为反转录转座，它需要转座因子编码的蛋白质。然而，逆转录转座并不仅仅由蛋白质完成，并且逆转录转座最有可能需要宿主编码的蛋白质。本研究发现LINEs的反转录转座中间体含有大量的宿主编码蛋白，可能参与了反转录转座过程。

项目成果

The zebrafish LINE, ZfL2-1, has a potential to obtain a novel coding sequence in its ORF1p coding region.

斑马鱼 LINE ZfL2-1 有可能在其 ORF1p 编码区获得新的编码序列。

• 发表时间: 2011
• 作者: K. Kawase;S. Hayashi;K. Takeya;Masaki Kajikawa
• 通讯作者: Masaki Kajikawa

The zebrafish LINE, ZfL2-1, has a potential to obtain a novel coding sequence in its ORF1p coding region

斑马鱼 LINE ZfL2-1 有潜力在其 ORF1p 编码区获得新的编码序列

• 发表时间: 2011
• 作者: S. Hayamizu;T. Furubayashi;T. Nakata;Masaki Kajikawa.
• 通讯作者: Masaki Kajikawa.

Self-Interaction, Nucleic Acid Binding, and Nucleic Acid Chaperone Activities Are Unexpectedly Retained in the Unique ORF1p of Zebrafish LINE

• DOI: 10.1128/mcb.06162-11
• 发表时间: 2012-01-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Nakamura, Mitsuhiro;Okada, Norihiro;Kajikawa, Masaki
• 通讯作者: Kajikawa, Masaki