Expression profiling of seed-specific pre-miRNA binding to Arabidopsis DCL1 protein

为了从拟南芥中分离到与DCL1结合的pre- mirna，我们制作了转基因拟南芥植株，表达DCL1与诱导启动子驱动的标签序列融合。我们用RT-PCR和western bolt分析证实了融合DCL1基因的表达水平。最后，我们利用免疫沉淀法从转基因植物中制备与融合的DCL1蛋白结合的pre- mirna

To isolate pre-miRNAs binding to DCL1 from Arabidopsis, we made transgenic Arabidopsis plants, which express the DCL1 fused with a tag sequence driven by the inducible promoter. We confirmed the expression level of the fusion DCL1 gene using RT-PCR and western bolt analyses. Finally, we are preparing the pre-miRNAs binding to the fused DCL1 protein from the transgenic plants by immunoprecipitation methods