Expression profiling of seed-specific pre-miRNA binding to Arabidopsis DCL1 protein

为了从拟南芥中分离与DCL 1结合的前体miRNA，我们制备了转基因拟南芥植物，其表达与由诱导型启动子驱动的标签序列融合的DCL 1。我们通过RT-PCR和Western blot分析证实了融合DCL 1基因的表达水平。最后，我们通过免疫沉淀的方法从转基因植物中制备与融合DCL 1蛋白结合的pre-miRNAs

To isolate pre-miRNAs binding to DCL1 from Arabidopsis, we made transgenic Arabidopsis plants, which express the DCL1 fused with a tag sequence driven by the inducible promoter. We confirmed the expression level of the fusion DCL1 gene using RT-PCR and western bolt analyses. Finally, we are preparing the pre-miRNAs binding to the fused DCL1 protein from the transgenic plants by immunoprecipitation methods