Analysis of membrane fusion process and related proteins on cytokinesis of brown algae

在这项研究中，通过电子断层扫描对白头翁受精卵进行了三维分析，以了解细胞质分裂过程中过渡膜的形态。分析表明，高尔基体衍生的囊泡与扁平的脑池(FCS)融合，形成了一个复杂的边缘结构(TA；管状附属物)。每个Fc由Tas连接，形成膜网络(MN)。胞质分裂晚期，MN长成宽膜囊(MS)。将3D图像转到任意角度，表明从MN到MSC的膜融合过程是分层的。此外，用双向凝胶电泳法(2-DE)分离了二裂叶藻受精卵的胞质分裂和菌丝体B处理受精卵的胞质分裂受阻蛋白。在凝胶上可重复分离的大约600个点中，来自胞质分裂阻断的受精卵的2-DE显示与对照相比31个低表达和2个高表达。

In this study, a three dimensional analysis was performed by electron tomography in zygotes of Silvetia babingtonii, for understanding the transitional membrane configuration during cytokinesis. The analysis revealed that a complicated architecture with fringes (TA; tubule-like appendage) were formed by fusion of Golgi derived vesicles to flat cisternae (FCs). Each FC was connected by TAs, thus forming the membranous network (MN). At advanced cytokinesis, the MN grew into broad membranous sac (MS). To turn the 3D image to arbitrary angle indicated a stratification of membrane fusion process from the MN to the MSc. Moreover, proteins extracted from zygotes of Fucus distichus on cytokinesis and cytokinesis blocked zygotes treated with latrunculin B were separated by two-dimensional gel electrophoresis (2-DE). From a total of about 600 spots reproducibly separated on gels, 2-DE from cytokinesis blocked zygotes showed 31 under-expressed and 2 over-expressed proteins in comparison with control.