Analysis of membrane fusion process and related proteins on cytokinesis of brown algae

本研究利用电子断层扫描技术对巴宾顿银草受精卵进行了三维结构分析，以了解胞质分裂过程中过渡膜的结构。分析表明，一个复杂的架构与条纹（TA;管状附属物）的融合高尔基体衍生的囊泡形成的扁平池（FC）。每个FC由TA连接，从而形成膜网络（MN）。在胞质分裂晚期，MN生长为宽膜囊（MS）。将3D图像旋转到任意角度表明从MN到MSc的膜融合过程分层。此外，提取的蛋白质的墨角双列墨角受精卵的胞质分裂和胞质分裂阻滞的受精卵用latrunculin B处理，分离的双向凝胶电泳（2-DE）。从共约600个点重复分离的凝胶，2-DE从胞质分裂阻滞受精卵显示31个低表达和2个过表达的蛋白质与对照相比。

In this study, a three dimensional analysis was performed by electron tomography in zygotes of Silvetia babingtonii, for understanding the transitional membrane configuration during cytokinesis. The analysis revealed that a complicated architecture with fringes (TA; tubule-like appendage) were formed by fusion of Golgi derived vesicles to flat cisternae (FCs). Each FC was connected by TAs, thus forming the membranous network (MN). At advanced cytokinesis, the MN grew into broad membranous sac (MS). To turn the 3D image to arbitrary angle indicated a stratification of membrane fusion process from the MN to the MSc. Moreover, proteins extracted from zygotes of Fucus distichus on cytokinesis and cytokinesis blocked zygotes treated with latrunculin B were separated by two-dimensional gel electrophoresis (2-DE). From a total of about 600 spots reproducibly separated on gels, 2-DE from cytokinesis blocked zygotes showed 31 under-expressed and 2 over-expressed proteins in comparison with control.