Analysis of membrane fusion process and related proteins on cytokinesis of brown algae

褐藻胞质分裂膜融合过程及相关蛋白分析

• 批准号: 21770065
• 负责人: NAGASATO Chikako
• 金额: $ 3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770065/
• 关键词: 植物形態; 褐藻類; 細胞質分裂; 膜融合; 微細構造; 細胞骨格; 褐藻

In this study, a three dimensional analysis was performed by electron tomography in zygotes of Silvetia babingtonii, for understanding the transitional membrane configuration during cytokinesis. The analysis revealed that a complicated architecture with fringes (TA; tubule-like appendage) were formed by fusion of Golgi derived vesicles to flat cisternae (FCs). Each FC was connected by TAs, thus forming the membranous network (MN). At advanced cytokinesis, the MN grew into broad membranous sac (MS). To turn the 3D image to arbitrary angle indicated a stratification of membrane fusion process from the MN to the MSc. Moreover, proteins extracted from zygotes of Fucus distichus on cytokinesis and cytokinesis blocked zygotes treated with latrunculin B were separated by two-dimensional gel electrophoresis (2-DE). From a total of about 600 spots reproducibly separated on gels, 2-DE from cytokinesis blocked zygotes showed 31 under-expressed and 2 over-expressed proteins in comparison with control.

在这项研究中，通过电子断层扫描进行了三维分析，以了解在细胞分裂过程中的过渡膜结构。分析表明，高尔基体衍生囊泡与扁平池（FCs）融合形成了复杂的带条纹结构（TA；管状附属物）。每个FC由TAs连接，形成膜性网络（MN）。细胞质分裂晚期，MN发育成宽膜囊。将三维图像转换为任意角度，表明从MN到MSc的膜融合过程分层。此外，利用二维凝胶电泳（2-DE）分离了经latrunculin B处理的受细胞质分裂和细胞质分裂阻断的合子中提取的蛋白质。在凝胶上可重复分离的约600个点中，来自细胞分裂阻断的受精卵的2- de与对照相比显示出31个低表达蛋白和2个高表达蛋白。

项目成果

電子線トモグラフィー法で見る褐藻エゾイシゲの細胞質分裂過程

电子断层扫描观察褐藻虾夷重的细胞分裂过程

• 发表时间: 2010
• 作者: 長里千香子;梶村直子;峰雪芳宣;本村泰三
• 通讯作者: 本村泰三

Novel proteins comprising the Stramenopile tripartite mastigoneme in Ochromonas danica(Chrisophyceae)

包含丹麦红藻（Chrisophyceae）中原生藻菌三部分鞭毛体的新蛋白质

• 发表时间: 2009
• 期刊: Journal of Phycology 45
• 作者: Yamagishi;T.;Motomura;T.;Nagasato;C.;Kawai;H.
• 通讯作者: H.

The Ectocarpusgenome and the independent evolution of multicellularity in the brown algae

Ectocarpus基因组和褐藻多细胞的独立进化

• 发表时间: 2010
• 期刊: Nature
• 影响因子: 64.8
• 作者: Cock;J.M.;Sterck;L.;Rouze;P.,Scornet;D.;Allen A.E.;Amoutzias;G.,Anthouard;V.;Artiguenave;F.;Aury,J.M.;Badger;J.H.;Beszteri;B.,Billiau;K.;Bonnet;E.;Bothwell,J.H.F.;Bowler;C.;Boyen;C.;Brownlee,C.;Carrano;C.J.;Charrier;B.;Cho,G
• 通讯作者: Cho,G

褐藻におけるplasmodesmataの微細構造解析

褐藻胞间连丝的超微结构分析

• 发表时间: 2010
• 作者: Y.Yawata;K.Toda;E.Setoyama;J.Fukuda;H.Suzuki;H.Uchiyama;N.Nomura;今岡進;寺内真・梶村直子・長里千香子・C.Katsaros・峰雪芳宣・奥田一雄・本村泰三
• 通讯作者: 寺内真・梶村直子・長里千香子・C.Katsaros・峰雪芳宣・奥田一雄・本村泰三

電子線トモグラフィー法で観る褐藻の細胞質分裂装置と原形質連絡構造

电子断层扫描观察褐藻细胞分裂器和胞间连丝结构

• 发表时间: 2011
• 作者: 長里千香子;寺内真;本村泰三
• 通讯作者: 本村泰三