Analysis of the infuluence of physiological and morphological variability to the synaptic integration of a nonspiking interneuron

本研究旨在利用神经生理学和计算方法研究生理形态变异对已识别的非尖峰中间神经元（LDS神经元）突触整合（突触输出）的影响。2009年，为了量化LDS细胞树突膜电导和三维结构的变异性，采用激光扫描显微镜对LDS细胞的膜电导进行了神经生理学和神经药理学研究，并对LDS细胞的三维结构进行了测量。结果显示细胞膜电导在细胞间有很大的可变性。然而，在使用荧光染料Lucifer Yellow记录细胞膜电导的同时，无法获得细胞的形态，因此无法从电生理记录的细胞中定量获得细胞的三维结构。2010年对LDS细胞的三种膜电导进行了定量估计。

The present study aimed to investigate the influence of physiological morphological variability to the synaptic integration (synaptic output) of an identified nonspiking interneuron (LDS neuron) using neurophysiological and computational methods. In 2009, in order to quantify the variability of the membrane conductance of dendrite and three-dimensional structure of the LDS cells, their membrane conductances were investigated neurophysiologically and neuropharmacologically and their three-dimensional structures were measured using a laser scanning microscope. The result shows large variability of the membrane conductance across the cells. However, the morphology of the cell could not be obtained while the membrane conductance was recorded at the same time using cell filling fluorescent dye, Lucifer Yellow so that three dimensional structures of the cells could not be quantified from the cell recorded electrophysiologically. In 2010, three types of membrane conductances in the LDS cell was estimated quantatively.