Identification of post-translational modification in nuclear proteins during neural stem cell fate specification by proteomic analysis

目前还没有完全理解神经干细胞的命运是如何指定的，尽管广泛的调查，例如，基因表达谱。为了解决这个重要的问题，我们集中在核蛋白的翻译后修饰的变化，如转录因子，辅激活子/辅抑制子，和染色质修饰剂在神经干细胞（NSC）响应成纤维细胞生长因子2（FGF 2），一个代表性的因素，以维持他们。神经干细胞刺激成纤维细胞生长因子2，其裂解物进行了检查，通过蛋白质组学分析。结果显示，在4095个点中，有80个点在FGF 2刺激后上调或下调，具有统计学显著差异。其中，我们集中在24个点差异磷酸化响应FGF 2，并确定了所有的nanoLC-QqTOF MS分析。这些蛋白质包含与核动力学相关的因子，包括核转位和染色质修饰。

It is not yet completely understood how neural stem cell fate is specified, in spite of extensive investigations by, for instance, gene expression profiling. To tackle this important question, we have focused on the changes in post-translational modification of nuclear proteins such as transcription factors, coactivators/corepressors, and chromatin modifiers in neural stem cells (NSCs) in response to fibroblast growth factor 2 (FGF2), a representative factor to maintain them. NSCs were stimulated by FGF2, and its lysates were examined by proteomic analysis. The result revealed 80 up- or down-regulated protein spots upon FGF2 stimulation out of 4095 spots with statistically significant differences. Among them, we focused on 24 spots differentially phosphorylated in response to FGF2, and identified all of them by nanoLC-QqTOF MS analysis. These proteins contained factors related to nuclear dynamics including nuclear translocation and chromatin modifications.