Investigation about the behavior of supramolecular complex in cell membrane : mechanism

细胞膜中超分子复合物行为的研究：机制

• 批准号: 21770157
• 负责人: FUKUOKA Hajime
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770157/
• 关键词: 分子モーター; イメージング; バクテリア; 一分子計測; イオン

Expression systems for GFP-FliN, FliM-GFP, and GFP-FliG were constructed, and the functional incorporation was confirmed. In a fluorescently labeled motor by GFP, the recovery of fluorescence after photobleaching by the evanescent light was observed. This is the first evidence for the exchange of rotor components in a functioning bacterial flagellar motor. The rotation of motors of individual E. coli cells by combining a high-speed camera and the bead assay method. From this experiment, it was showed that the switching of two different motors on a cell is controlled coordinatedly by an intracellular signal protein, phosphorylated CheY. From these results, wave-like propagation model of intracellular signal was proposed.

构建了 GFP-FliN、FliM-GFP 和 GFP-FliG 的表达系统，并确认了功能整合。在 GFP 荧光标记的电机中，观察到倏逝光光漂白后荧光的恢复。这是功能性细菌鞭毛马达中转子部件交换的第一个证据。通过结合高速相机和珠分析方法来旋转单个大肠杆菌细胞的电机。该实验表明，细胞上两个不同马达的切换是由细胞内信号蛋白磷酸化 CheY 协调控制的。根据这些结果，提出了细胞内信号的波状传播模型。

项目成果

Role of the polar localization of CheZ for the propagation of intracellular signal in bacterial chemotaxis system

CheZ 极性定位对细菌趋化系统细胞内信号传播的作用

• 发表时间: 2012
• 作者: Fukuoka;H.
• 通讯作者: H.

Coordinated Regulation of Multiple Locomotive apparatus in a Single Bacterial Cell

单个细菌细胞中多个机车装置的协调调节

• 发表时间: 2012
• 作者: Hajime Fukuoka;Takashi Sagawa;Yuichi Inoue;Hiroto Takahashi;Akihiko Ishijima
• 通讯作者: Akihiko Ishijima

Coordinated regulation of multiple flagellar motors by the Escherichia coli chemotaxis system. Biophysics

大肠杆菌趋化系统对多个鞭毛马达的协调调节。

• 发表时间: 2012
• 期刊: Biophysics
• 作者: Fukuoka;H.;Inoue;Y.;Ishijima;A.
• 通讯作者: A.

Coordinated regulation of flagellar motors on a single Escherichia coli cell

单个大肠杆菌细胞鞭毛马达的协调调节

• 发表时间: 2011
• 作者: Fukuoka;H.;Terasawa;S.;Inoue;Y.;Sagawa;T.;Takahashi;H.;Ishijima;A.
• 通讯作者: A.

Coordinated Regulation of Multiple Locomotive

多机车协调调控

• 发表时间: 2012
• 作者: Fukuoka;H.;Sagawa;T.;Inoue;Y.;Takahashi;T.;Ishijima;A.
• 通讯作者: A.