Investigation about the behavior of supramolecular complex in cell membrane : mechanism

构建了GFP-FliN、FliM-GFP和GFP-FliG的表达系统，并确认了功能性掺入。在GFP荧光标记的马达中，观察到渐逝光光漂白后荧光的恢复。这是第一个证据交换转子组件在一个功能细菌鞭毛马达。单个E.大肠杆菌细胞通过结合高速摄像机和珠测定方法。从这个实验中，它表明，在一个细胞上的两个不同的马达的开关是由细胞内的信号蛋白，磷酸化的CheY协调控制。在此基础上，提出了细胞内信号的类波传播模型。

Expression systems for GFP-FliN, FliM-GFP, and GFP-FliG were constructed, and the functional incorporation was confirmed. In a fluorescently labeled motor by GFP, the recovery of fluorescence after photobleaching by the evanescent light was observed. This is the first evidence for the exchange of rotor components in a functioning bacterial flagellar motor. The rotation of motors of individual E. coli cells by combining a high-speed camera and the bead assay method. From this experiment, it was showed that the switching of two different motors on a cell is controlled coordinatedly by an intracellular signal protein, phosphorylated CheY. From these results, wave-like propagation model of intracellular signal was proposed.