Identification of torque generation part in flagellar motor by using fluorescence polarization

荧光偏振识别鞭毛马达扭矩产生部分

• 批准号: 24770144
• 负责人: FUKUOKA Hajime
• 金额: $ 3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770144/
• 关键词: バイオイメージング; 分子モーター; べん毛モーター

The microscopic system to detect the polarization of fluorescent molecules was develoved. By using this microscopic system, the polarization of fluorescence from a single GFP molecule was detected, and the direction of a GFP molecule on the surface of coverslip was estimated from the fluorescent intensities of GFP in P-polarization and S-polarization. The microscopic system to simultaneously observe the fluorescence from GFP-fusion proteins and the rotation of flagellar motor was also constructed. By using this microscopic system, it was demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of phosphorylated CheY by simultaneously visualizing CheY tagged with GFP and the rotational switching of a motor in live cells. The results of this study was published from Sci. Signal. 2014. 7: ra32.

研制了一种用于检测荧光分子偏振的显微系统。通过使用该显微系统，检测来自单个GFP分子的荧光偏振，并且根据GFP在P偏振和S偏振中的荧光强度来估计GFP分子在盖玻片表面上的方向。构建了同时观察GFP融合蛋白荧光和鞭毛马达旋转的显微系统。通过使用该显微镜系统，通过同时可视化用GFP标记的CheY和活细胞中马达的旋转开关，证明马达的旋转开关直接由磷酸化CheY的结合和解离调节。这项研究的结果发表在Sci。信号了2014. 7：ra32.

项目成果

Direct Imaging of Intracellular Signaling Components That Regulate Bacterial Chemotaxis

• DOI: 10.1126/scisignal.2004963
• 发表时间: 2014-04-01
• 期刊: SCIENCE SIGNALING
• 影响因子: 7.3
• 作者: Fukuoka, Hajime;Sagawa, Takashi;Ishijima, Akihiko
• 通讯作者: Ishijima, Akihiko

蛍光イメージングによる大腸菌細胞内情報伝達の直接的観察

使用荧光成像直接观察大肠杆菌的细胞内通讯

• 发表时间: 2013
• 作者: 上野裕則;ブイフイ;石川尚;石川拓司;山口隆美;福岡創
• 通讯作者: 福岡創

複数べん毛モーターの同時計測による,大腸菌細胞内シグナル伝達の計測

通过同时测量多个鞭毛马达来测量大肠杆菌的细胞内信号转导

• 发表时间: 2012
• 作者: H. Fukuoka;T. Sagawa;Y. Inoue;H. Takahashi;A. Ishijima;福岡創,佐川貴志,高橋泰人,井上裕一,石島秋彦
• 通讯作者: 福岡創,佐川貴志,高橋泰人,井上裕一,石島秋彦

Direct imaging of CheY-binding to a functioning bacterial flagellar motor

CheY 与功能性细菌鞭毛运动结合的直接成像

• 发表时间: 2013
• 作者: H. Fukuoka;T. Sagawa;Y. Inoue;H. Takahashi;A. Ishijima
• 通讯作者: A. Ishijima

Propagation of intracellular signaling molecule in the presence and absence of polar localization of CheZ in a single E. coli cell

在单个大肠杆菌细胞中存在和不存在 CheZ 极性定位的情况下胞内信号分子的传播

• 发表时间: 2012
• 作者: H. Fukuoka;T. Sagawa;Y. Inoue;H. Takahashi;A. Ishijima
• 通讯作者: A. Ishijima