Analyses of the regulation of the chromatin structure and transcriptional activity using in vitro reconstitution system

为了揭示由特定因素如组蛋白尾修饰或染色体蛋白诱导的结构，我们观察了在各种条件下重建的染色质。(1)组蛋白尾部的超乙酰化导致核小体和较细的H1诱导纤维之间的排斥力比非乙酰化的染色质更强。(2)用快速原子力显微镜观察到核小体的滑动和解离。(3)ORC（origin recognition complex）与核小体结合形成更大的复合体。

In order to reveal the structure induced by specific factors such as histone-tail modifications or chromosomal proteins, we observed the reconstituted chromatin under the various conditions.(1) hyper-acetylation of histone-tails causes stronger repulsive force between nucleosomes and the thinner H1-induced fiber than non-acetylated chromatin.(2) Sliding and dissociation of nucleosomes were observed by fast-AFM.(3) ORC(origin recognition complex) associated with nucleosome and formed larger complex.