Analyses of the regulation of the chromatin structure and transcriptional activity using in vitro reconstitution system

利用体外重构系统分析染色质结构和转录活性的调控

• 批准号: 21770167
• 负责人: HIZUME Kohji
• 金额: $ 2.91万
• 依托单位: National Institute of Genetics (2010-2011)Kyoto University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770167/
• 关键词: タンパク質; 核酸の構造・動態・機能; クロマチン; ヌクレオソーム; 原子間力顕微鏡; DNA複製; 原子力間顕微鏡; 試験管内再構成

In order to reveal the structure induced by specific factors such as histone-tail modifications or chromosomal proteins, we observed the reconstituted chromatin under the various conditions.(1) hyper-acetylation of histone-tails causes stronger repulsive force between nucleosomes and the thinner H1-induced fiber than non-acetylated chromatin.(2) Sliding and dissociation of nucleosomes were observed by fast-AFM.(3) ORC(origin recognition complex) associated with nucleosome and formed larger complex.

为了揭示组蛋白尾部修饰或染色体蛋白等特定因素诱导的结构，我们观察了不同条件下重组的染色质。(1)组蛋白尾部的超乙酰化使核小体与较薄的h1诱导纤维之间的斥力比非乙酰化的染色质更强。(2)快速原子力显微镜观察核小体的滑动和解离。(3) ORC（origin recognition complex）与核小体结合形成更大的复合物。

项目成果

Molecular dynamics of DNA and nucleosomes in solution studied by fast-scanning atomic force microscopy

• DOI: 10.1016/j.ultramic.2010.02.032
• 发表时间: 2010-05-01
• 期刊: ULTRAMICROSCOPY
• 影响因子: 2.2
• 作者: Suzuki, Yuki;Higuchi, Yuji;Takeyasu, Kunio
• 通讯作者: Takeyasu, Kunio

Single-molecule anatomy by atomic force microscopy and recognition imaging

通过原子力显微镜和识别成像进行单分子解剖

• 发表时间: 2009
• 期刊: Arch Histol Cytol
• 作者: Takahashi;H.;Hizume;K.;Kumeta;M.;S;H. Y.;and Takeyasu;K.
• 通讯作者: K.

Nano-scale analysis of the chromatin decompaction induced by histone acetylation

组蛋白乙酰化引起的染色质解压缩的纳米级分析

• 发表时间: 2011
• 期刊: Archives of Histology and Cytology
• 作者: Hizume;K.;Araki;S.;Hata;K.;Prieto;E.;Kundu;T. K.;Yoshikawa;K.;and Takeyasu;K.;野中茂紀;Kohji Hizume
• 通讯作者: Kohji Hizume

Structural properties of yeast chromatin fiber examined by AFM and in vitro reconstitution system

AFM 和体外重构系统检测酵母染色质纤维的结构特性

• 发表时间: 2009
• 期刊: Proceedings of 2009 International Symposium on Micro-Nano Mechatronics and Human Science
• 作者: Prieto;E.;Hizume;K.;Takeyasu;K.;and Yoshimura;S. H.
• 通讯作者: S. H.

Nano-scale analyses of the chromatin decompaction induced by histone acetylation

组蛋白乙酰化诱导的染色质解压缩的纳米级分析

• 发表时间: 2011
• 期刊: Arch Histol Cytol
• 作者: Hizume;K.;Araki;S.;Hata;K.;Prieto;E.;Kundu;T. K.;Yoshikawa;K.;and Takeyasu;K.
• 通讯作者: K.