Involvement of the Cdc7 protein kinse in sister chromatid cohesion

染色体的忠实复制和分离是细胞增殖的必要条件。复制的姐妹染色体在合成过程中是物理连接的（姐妹染色单体内聚），确保染色体对的识别被分配到子细胞中。虽然内聚建立反应与DNA复制密切相关，但内聚与复制耦合的机制尚不清楚。在这项研究中，我们研究了DNA复制蛋白如何与爪蟾卵提取物的内聚蛋白相互作用。

Faithful duplication and segregation of chromosomes are essential for cell proliferation. Replicated sister chromosomes are physically linked during their synthesis (sister chromatid cohesion), ensuring identification of chromosome pairs to be distributed into daughter cells. Although the cohesion establishment reaction cooperates with DNA replication, the mechanism how cohesion and replication couple remains unclear. In this study, we have examined how a DNA replication protein interacts with a cohesion protein using Xenopus egg extracts.