Experimental validation of the significance of and molecular mechanism for gene amplification phenomena

基因扩增涉及多种生物现象，包括恶性进展、耐药性和基因进化。然而，分子机制的细节仍有待确定。基于我们对酵母基因扩增系统的发现，我们为阐明癌基因扩增的机制提供了线索。我们还研究了标记基因的构建，以灵敏地检测基因扩增中涉及的突变的引入。

Gene amplification is involved in a variety of biological phenomena, including malignant progression, drug resistance, and gene evolution. However, details of the molecular mechanisms remain to be determined. Based on our findings of gene amplification system in yeast, we have obtained a clue to elucidate the mechanism for oncogene amplification. We also work on the construction of marker gene that sensitively detect the introduction of mutations involved in gene amplification.