Identification of carbohydrates on the surface of fungal vector that fungal-borne viurs specifically attach.

用热酚法从橄榄藻游动孢子中分离出一种吸附病毒的提取物，用薄层色谱和SDS-PAGE均未分离出该提取物。说明提取物中含有多糖。该提取物与ConA有较强的反应。ConA也在游动孢子的表面发生反应，表明游动孢子表面存在物质。当通过凝胶过滤分析推测分子量时，它们含有一些不同分子量的底物，这取决于分离物。这表明这种差异与真菌传播的特异性有关。

The extract, which was isolated from zoospores of olpidium by the hot phenol method and absorbed a virus, were not separated by Thin-Layer Chromatography and SDS-PAGE. It suggests that the extract includes polysaccharide. The extract strongly reacted to Concanavalin A(ConA). The ConA also reacted the surface of zoospores, suggesting that substances exist on the surface of zoospores. When the molecular weight was presumed by gel filtration analysis, they contained some substrates with different high molecular weights that depend on isolates. It suggested that this difference is involved in specificity of fungal transmission.