Localization of Candidatus Liberibacter asiaticus in Diaphorina citri(Hemiptera : Psyllidae)

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Diaphorina citri will not always transmit Candidatus Liberibacter asiaticus(Las), the bacterium associated with the disease of huanglongbing or citrus greening, if the vector fed on infected citrus tree. We investigated the localization of Las in Las-positive but non-transmissible D. citri adults by oligonucleotide-probed in situ hybridization targeted to the bacterial 16S ribosomal RNA. The significant signals showing the targets were detected in the filter chamber, midgut, ovalies in tissue sections of non-transmissible Las-positive D. citri.