Fermentative production of plant alkaloids in L-tyrosine producing Escherichia coli.

我们通过两个步骤的基因工程获得了一株高产L酪氨酸的大肠杆菌菌株。一株大肠杆菌扰乱了tyrR基因，该基因的产物抑制了与芳香氨基酸生物合成有关的基因的表达。此外，反馈型抑制抗性(FBR)3-脱氧-D-阿拉伯-七磺酸盐-7-磷酸合成酶、FBR-分支酸变位酶/预苯酸脱氢酶、磷酸烯醇式丙酮酸合成酶和转酮醇酶在一株干扰TyrR基因的菌株中过表达。在L酪氨酸高产菌株中引入青枯菌酪氨酸酶、恶臭假单胞菌多巴脱羧酶和多巴胺网状生物合成酶，建立了以简单碳源生产植物生物碱的大肠杆菌发酵体系。这里描述的发酵平台为低成本生产多种生物碱提供了机会。

We generated an E. coli strain that over-produces L-tyrosine by two steps of genetic engineering. An E. coli strain has disrupted the tyrR gene, the product of which represses the expression of genes involved in aromatic amino acid biosynthesis. In addition, the feedback-inhibition-resistant (fbr) 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, fbr-chorismate mutase/prephenate dehydrogenase, phosphoenolpyruvate synthetase, and transketolase are over-expressed in an E. coli strain disrupted the tyrR gene. We establish an E. coli fermentation system that yields plant alkaloids from simple carbon sources, introducing tyrosinase from Ralstonia solanacearum, DOPA decarboxylase from Pseudomonas putida, and reticuline biosynthetic enzymes from dopamine in the L-tyrosine over-producing E. coli strain. The fermentation platform described here offers opportunities for low-cost production of many diverse alkaloids.