Genetic diversity and population history of cold temperate pine species in north-east Asia

东北亚冷温带松树种的遗传多样性和种群历史

• 批准号: 21780141
• 负责人: AIZAWA Mineaki
• 金额: $ 1.66万
• 依托单位: Utsunomiya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780141/
• 关键词: 遺伝的多様性; 第四紀; チョウセンゴヨウ; 分布変遷; 北東アジア; ミトコンドリアDNA; 葉緑体DNA; 核DNA; SSRマーカー; 系統地理; マツ科

This study aimed to delineate the population history during the Quaternary of cold temperate pine, Korean pine (Pinus koraiensis), in north-east Asia. Range-wide genetic variation was assessed using mitochondrial DNA (mtDNA) and chloroplast DNA across the entire distribution range of the species. Genetic diversity of mtDNA haplotypes showed a complete loss of the diversity across the continental populations (H_T, total gene diversity, =0) exhibiting a single haplotype in spite of the extensive species natural range with large-sized populations. In contrast, a higher level of genetic diversity was observed in Japanese populations (H_T=0.502) harbouring three mtDNA haplotypes despite the restricted natural range with quite small populations. The loss of genetic diversity and fixation of the single haplotype in the continental populations could result from the contraction into a single refugium in the past ; the higher level of genetic diversity of Japanese populations suggested that Korean pine retained large-sized populations in Japan during the Pleistocene.

本研究旨在描述东北亚寒温带红松(Pinus Koraiensis)第四纪的种群历史。使用线粒体DNA(MtDNA)和叶绿体DNA评估了该物种整个分布范围内的广泛遗传变异。线粒体DNA单倍型的遗传多样性显示大陆种群的多样性(H_T，总基因多样性=0)完全丧失，呈现单一单倍型，尽管种群规模大，物种自然范围广泛。相反，拥有三种线粒体DNA单倍型的日本种群(H_T=0.502)具有较高水平的遗传多样性，尽管种群数量很小，但其自然分布范围有限。大陆种群遗传多样性的丧失和单一单倍型的固定可能是由于过去收缩为单一的避难所所致；日本种群较高的遗传多样性水平表明更新世红松在日本保留了大量的种群。