Molecular biological study on the pathogenic nematode-host plant interactions at the early stage of pine wilt disease

松材线虫病早期病原线虫与寄主植物相互作用的分子生物学研究

• 批准号: 21780147
• 负责人: TAKEUCHI Yuko
• 金额: $ 3.08万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780147/
• 关键词: 遺伝子発現; 森林病害; 線虫; 相互認識機構; プロテオーム; マツノザイセンチュウ

Pine wilt disease is an infectious forest disease caused by the pathogenic pine wood nematode, Bursaphelenchus xylophilus. To elucidate the pathogenic mechanism of the disease, the interaction between the nematode and host pine at an early stage was investigated, by means of molecular biological techniques. The proteomic analyses determined the profile of surface-coat proteins and secreted proteins of the nematode, and cDNA subtraction analysis determined several genes involved in the host responses. Results obtained indicate that the pine wood nematode has a kind of system to defeat host defense responses.

松材线虫病是由松材线虫松材线虫引起的一种森林传染病。为了阐明该病害的致病机制，利用分子生物学技术对早期线虫与寄主松树之间的相互作用进行了研究。蛋白质组分析确定了线虫表面外壳蛋白和分泌蛋白的概况，cDNA 消减分析确定了与宿主反应有关的几个基因。获得的结果表明松材线虫具有一种击败宿主防御反应的系统。

项目成果

病原力の異なるマツノザイセンチュウ4系統間での比較セクレトーム解析

四种不同致病力线虫菌株分泌组比较分析

• 发表时间: 2010
• 作者: Yuko Takeuchi;Ryoji Shinya;Keita Ichimura;Shuhei Takemoto;Kazuyoshi Futai;新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
• 通讯作者: 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎

Surface coat and secretion proteins of the pine wood nematode : the strategy requirements for attack and defense at the host-parasite interface

松材线虫的表面外壳和分泌蛋白：宿主-寄生虫界面攻击和防御的策略要求

• 发表时间: 2011
• 作者: Shinya;R.;Takeuchi;Y.;Morisaka;H.;Ueda;M.;and Futai;K
• 通讯作者: K

Establishment of the genetically uniformed pure-lines of the pine wod nematode, Bursaphelenchus xylophilus

松材线虫松材线虫遗传均一纯系的建立

• 发表时间: 2010
• 作者: Yuko Takeuchi;Ryoji Shinya;Keita Ichimura;Shuhei Takemoto;Kazuyoshi Futai
• 通讯作者: Kazuyoshi Futai