Preparation of an active recombinant peptide of crustacean androgenic gland hormone, a heterodimeric glycopeptide.

甲壳类雄激素活性重组肽（异二聚糖肽）的制备。

• 批准号: 21780186
• 负责人: OHIRA Tsuyoshi
• 金额: $ 2.91万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780186/
• 关键词: 造雄腺ホルモン; オカダンゴムシ; オニテナガエビ; 十脚目; 等脚目; 甲殻類; 性転換; 性分化

The sex differentiation in crustaceans is known to be controlled by a peptide hormone called androgenic gland hormone (AGH). The primary structure of the terrestrial isopod Armadillidium vulgare AGH (Arv-AGH) was finally determined to be a heterodimeric glycoprotein. An N-linked glycan moiety in the mature Arv-AGH was found to be essential for biological activity. Therefore, in this study, a recombinant AGH with a glycan moiety was produced using a baculovirus expression system. Insect Sf9 cells were infected with a recombinant baculovirus expression vector containing an Arv-AGH cDNA insert and subsequently recombinant Arv-AGH was expressed. In Western blot analysis using an anti-Arv-AGH antibody, immunoreactive band of a glycosylated recombinant Arv-AGH was detected.Finally, a cDNA encoding AGH-like peptide (Mar-IAG) was cloned from the giant freshwater prawn Macrobrachium rosenbergii and subsequently a recombinant Mar-IAG with a glycan moiety was expressed by the same methods as recombinant Arv-AGH.

甲壳类动物的性别分化是由一种叫做雄激素腺激素（AGH）的肽激素控制的。陆生等足动物Armadillidium vulgare AGH （Arv-AGH）的初级结构最终确定为异二聚体糖蛋白。在成熟的Arv-AGH中发现一个n -链聚糖片段对生物活性至关重要。因此，本研究利用杆状病毒表达系统制备了含有多糖片段的重组AGH。用含有Arv-AGH cDNA插入片段的重组杆状病毒表达载体感染昆虫Sf9细胞，表达重组Arv-AGH。使用抗Arv-AGH抗体进行Western blot分析，检测到糖基化重组Arv-AGH的免疫反应带。最后，从罗氏沼虾（Macrobrachium rosenbergii）中克隆了一个编码agh样肽（Mar-IAG）的cDNA，并通过与重组Arv-AGH相同的方法表达了含有糖基片段的重组Mar-IAG。

项目成果

発生に伴うオカダンゴムシ造雄腺ホルモン(AGH)の発現動態の解析

药丸虫发育过程中雄激素（AGH）表达动态分析

• 发表时间: 2010
• 期刊: Sci. J. Kanagawa Univ. Vol.21
• 作者: 泉進;齊藤裕;長澤寛道;長谷川由利子;大平剛
• 通讯作者: 大平剛

オニテナガエビの造雄腺ホルモン様分子に対するポリクローナル抗体の作製

虾雄激素样分子多克隆抗体的制备

• 发表时间: 2010
• 作者: 大平剛;坂西綱太;鶴岡慎哉;鶴岡慎哉
• 通讯作者: 鶴岡慎哉

バキュロウイルス発現系を用いた組換えオニテナガエビ造雄腺ホルモン様分子の作製

利用杆状病毒表达系统制备重组雄激素样分子

• 发表时间: 2010
• 作者: 大平剛;坂西綱太;鶴岡慎哉;鶴岡慎哉;坂西綱太
• 通讯作者: 坂西綱太

成長に伴うオニテナガエビ造雄腺の組織像の変化

O. nigra 雄激素腺生长过程中组织学的变化

• 发表时间: 2010
• 作者: 大平剛;坂西綱太;鶴岡慎哉
• 通讯作者: 鶴岡慎哉

Molecular cloning of a cDNA encoding insulin-like androgenic gland factor from the kuruma prawn Marsupenaeus japonicus and analysis of its expression

• DOI: 10.1007/s12562-011-0337-8
• 发表时间: 2011-03
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: Kota Banzai;N. Ishizaka;K. Asahina;Katsuyoshi Suitoh;S. Izumi;T. Ohira