Functional Analysis of Nucleoplasmin in Mammalian Oocyte Nucleolus

在哺乳动物中，卵母细胞核仁是早期胚胎发育所必需的，但卵母细胞核仁的功能难以捉摸。详细的分析表明，缺乏核仁的受精卵表现为精子染色质解聚延迟，间期结构性异染色质解聚，第一次有丝分裂进程缺陷。这些结果表明，卵母细胞核仁参与了受精卵的功能性染色质组织。此外，我们还鉴定了卵母细胞核仁中与核浆蛋白2(Npm2)相互作用的蛋白质。这些蛋白质的功能正在分析中。

In mammals, the oocyte nucleolus is required for early embryonic development, but the function of oocyte nucleolus is elusive. Detailed analysis revealed that the zygotes lacking nucleolus showed the delay of sperm chromatin decondensation, constitutive heterochromatin disorganization during interphase, and the defect in first mitotic progression. These results indicate that the oocyte nucleolus is involved in the functional chromatin organization in zygotes. Moreover, we identified the proteins interacted with nucleoplasmin2 (Npm2) in the oocyte nucleolus. The function of these proteins is under analysis.