Obstruction of HA antigen cleavage of influenza virus with recombinant H chain antibody and its antiviral effects

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It is a research that develops H chain antibody of the camel family animal, reported to have an excellent antigen specificity and compatibility, to control influenza virus HA protein effectively, and that provide the treatment model of the virus disease. The H chain antibodies were separated from the llama immunized with HA antigen, and were reactive to the HA antigen after the treat for 30 minutes at 90℃. The amplified VHH antibody gene PCR products were introduced into phagemid vectors and separated from transformed DH5α. It is necessary to examine the utility of the gene restructuring, by an artificial mutation introduction or the DNaseI processing, to isolate the VHH clone with a stronger affinity.