Obstruction of HA antigen cleavage of influenza virus with recombinant H chain antibody and its antiviral effects

重组H链抗体阻断流感病毒HA抗原裂解及其抗病毒作用

• 批准号: 21780280
• 负责人: LEE Sung-Il
• 金额: $ 2.33万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780280/
• 关键词: インフルエンザウイルス; H鎖抗体; 人獣共通感染症

It is a research that develops H chain antibody of the camel family animal, reported to have an excellent antigen specificity and compatibility, to control influenza virus HA protein effectively, and that provide the treatment model of the virus disease. The H chain antibodies were separated from the llama immunized with HA antigen, and were reactive to the HA antigen after the treat for 30 minutes at 90℃. The amplified VHH antibody gene PCR products were introduced into phagemid vectors and separated from transformed DH5α. It is necessary to examine the utility of the gene restructuring, by an artificial mutation introduction or the DNaseI processing, to isolate the VHH clone with a stronger affinity.

这是一项研究开发骆驼科动物的H链抗体，据报道具有良好的抗原特异性和相容性，以有效控制流感病毒HA蛋白，并提供了病毒病的治疗模式。从HA抗原免疫的大羊驼中分离出H链抗体，并在90℃时与HA抗原反应30min。将扩增的VHH抗体基因扩增产物导入噬菌体载体，从转化的α中分离。为了分离亲和力更强的VHH克隆，有必要通过人工诱变或DNaseI处理来检测基因重组的实用性。

项目成果

Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats

RB51株疫苗对Sprague-Dawley大鼠流产布鲁氏菌感染和垂直传播的保护作用

• 发表时间: 2009
• 期刊: Journal of Veterinary Science 10
• 作者: Islam;M.A.;Khatun;M.M.;Baek;B.-K.;Lee;S.-I.
• 通讯作者: S.-I.

Construction of artificial promoters senistivity responsive to sonication in vitro.

体外超声处理敏感性人工启动子的构建。

• 发表时间: 2009
• 期刊: Journal of Medical Ultrasonics 36
• 作者: Akihiko Watanabe;Satoshi Kakutani;Ryohei Ogawa;Sung-il Lee;Toru Yoshida;Akihiro Morii;Go Kagiya;Loreto B.Feril Jr.;Hideki Fuse;Takashi Kondo
• 通讯作者: Takashi Kondo

Cellular and humoral immune responses and antigen recognition in Sprague-Dawley rats experimentally infected with Brucella abortus biotype 1

实验性感染流产布鲁氏菌生物型 1 的 Sprague-Dawley 大鼠的细胞和体液免疫反应以及抗原识别

• 发表时间: 2009
• 期刊: Asian Journal of Animal and Veterinary Advances 4
• 作者: Khatun;M.M.;Islam;M.A.;Baek;B.-K.;Lee;S.-I.
• 通讯作者: S.-I.

Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats.

RB51 株疫苗在预防 Sprague-Dawley 大鼠流产布鲁氏菌感染和垂直传播方面的功效。

• 发表时间: 2009
• 期刊: Journal of Veterinary Science 10
• 作者: Md.Ariful Islam;Mst.Minara Khatun;Byeong-kirl Baek;Sung-il Lee
• 通讯作者: Sung-il Lee

Cellular and humoral immune responses and antigen recognition in Sprague-Dawleyrats experimentally infected with Brucella abortus biotype 1.

实验感染流产布鲁氏菌生物型 1 的 Sprague-Dawleyrats 的细胞和体液免疫反应以及抗原识别。

• 发表时间: 2009
• 期刊: Asian Journal of Animal and Veterinary Advances 4
• 作者: Mst.Minara Khatun;Md.Ariful Islam;Byeong-kirl Baek;Sung-il Lee
• 通讯作者: Sung-il Lee