Development of oral inactivated recombinant Salmonella vaccine using E.coli cell surface display technique
利用大肠杆菌细胞表面展示技术开发口服灭活重组沙门氏菌疫苗
基本信息
- 批准号:21780290
- 负责人:
- 金额:$ 2.75万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Young Scientists (B)
- 财政年份:2009
- 资助国家:日本
- 起止时间:2009 至 2012
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In order to develop oral inactivated Salmonella Enteritidisvaccine, tne E. coli surface display plasmid vector was newly constructed in this study. The N-terminal domain of MisL, which is classified as Salmonella type V secretion system, was employed an anchor protein for surface display of recombinant protein. The fusion proteins of fully functional cholera toxin B subunit (CTB), which is one of the suitable candidates for mucosal adjuvant, and Salmonella Enteritidis vaccine candidate proteins were able to be displayed on the surface of E. coli. The chickens were immunized orally with the inactivated E. coli expressed fusion proteins. The CTB-specific IgA titers in intestinal mucus were enhanced. Our findings obtained in this study can be applied to development of simple and safety oral SE vaccine.
为了研制口服肠炎沙门氏菌灭活疫苗,本研究构建了大肠杆菌表面展示质粒载体。MisL的n端结构域被归类为沙门氏菌V型分泌系统,作为锚定蛋白用于重组蛋白的表面展示。全功能霍乱毒素B亚基(CTB)与肠炎沙门氏菌疫苗候选蛋白的融合蛋白能够在大肠杆菌表面显示。用灭活的大肠杆菌表达的融合蛋白口服免疫鸡。肠道黏液中ctb特异性IgA滴度增强。本研究结果可用于研制简单、安全的口服SE疫苗。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan.
隐孢子虫和贾第鞭毛虫属的调查和分子特征。
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:2.6
- 作者:Yoshiuchi R;Matsubayashi M;Kimata I;Furuya M;Tani H;Sasai K.
- 通讯作者:Sasai K.
Coprological Survey of Parasitic Infections in Pigs and Cattle in Slaughterhouse in Osaka, Japan
- DOI:10.1292/jvms.71.1079
- 发表时间:2009-08-01
- 期刊:
- 影响因子:1.2
- 作者:Matsubayashi, Makoto;Kita, Toshimasa;Baba, Eiichiroh
- 通讯作者:Baba, Eiichiroh
Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice.
低 pH 对安氏隐孢子虫子孢子形态和活力以及感染小鼠胃中组织病理学的影响。
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:4
- 作者:Matsubayashi M;Ando H;Kimata I;Takase H;Nakagawa H;Furuya M;Tani H;Sasai K.
- 通讯作者:Sasai K.
Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media
- DOI:10.1017/s0031182010000685
- 发表时间:2010-11-01
- 期刊:
- 影响因子:2.4
- 作者:Matsubayashi, M.;Ando, H.;Sasai, K.
- 通讯作者:Sasai, K.
Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan
- DOI:10.1016/j.vetpar.2010.11.008
- 发表时间:2011-03-10
- 期刊:
- 影响因子:2.6
- 作者:Matsubayashi, Makoto;Carreno, Ramon A.;Sasai, Kazumi
- 通讯作者:Sasai, Kazumi
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TANI Hiroyuki其他文献
TANI Hiroyuki的其他文献
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{{ truncateString('TANI Hiroyuki', 18)}}的其他基金
Development of a novel vaccine platform for chickens using bacterial outer membrane vesicles
利用细菌外膜囊泡开发新型鸡疫苗平台
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19K06388 - 财政年份:2019
- 资助金额:
$ 2.75万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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