Development of oral inactivated recombinant Salmonella vaccine using E.coli cell surface display technique

点击翻译按钮获取中文摘要

In order to develop oral inactivated Salmonella Enteritidisvaccine, tne E. coli surface display plasmid vector was newly constructed in this study. The N-terminal domain of MisL, which is classified as Salmonella type V secretion system, was employed an anchor protein for surface display of recombinant protein. The fusion proteins of fully functional cholera toxin B subunit (CTB), which is one of the suitable candidates for mucosal adjuvant, and Salmonella Enteritidis vaccine candidate proteins were able to be displayed on the surface of E. coli. The chickens were immunized orally with the inactivated E. coli expressed fusion proteins. The CTB-specific IgA titers in intestinal mucus were enhanced. Our findings obtained in this study can be applied to development of simple and safety oral SE vaccine.