Establishment of feline embryonic stem cell derived from developmental engineering
Establishment of feline embryonic stem cell derived from developmental engineering
批准号:
21780289
负责人:
HATOYA Shingo
金额:
$2.91万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2010
中文摘要
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英文摘要
The objective of this study was to establish ES cell lines from the cat for regenerative veterinary medicine. I examined feline IVM/IVF/IVC and establishment of feline embryonic stem cells, and the following results were obtained.(1)The BCB(+) oocytes reached more MII and morula/blastocysts than BCB (-) oocytes.(2)The isolation of feline sperm by percoll density gradient is effective in selecting the useful sperm.(3)Sequence alignment and comparison of feline LIF cDNA with human and mouse LIF cDNA revealed a high degree of nucleotide similarity.(4)The IVC of Only-one medium+FBS developed the feline embryo up to blastocyst stage.(5)Primary feline ES-like cell colonies were formed from IVF derived blastocysts.
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サルES細胞からのオリゴデンドロサイトへの簡便な分化誘導法
一种诱导猴 ES 细胞分化为少突胶质细胞的简单方法
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[瀧野麻衣亜, 奥野剛, 鳩谷晋吾, 大村雅, 鳥居隆三, 杉浦喜久弥, 玉田尋通, 川手憲俊, 高橋正弘, 稲葉俊夫]
通讯作者:
稲葉俊夫
再生医療の最前線~獣医臨床応用に向けた課題~「幹細胞:犬ES細胞(胚性幹細胞)の開発」
再生医学最前沿-兽医临床应用的挑战-《干细胞:犬ES细胞(胚胎干细胞)的开发》
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[寺西美佳, 高橋正明, 中村憲太郎, 古川晴也, 日出間純, 道下正貴, 鳩谷晋吾]
通讯作者:
鳩谷晋吾
Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas.
正常和隐睾小型腊肠犬和吉娃娃雌激素受体 1 基因 3 区域单核苷酸多态性分析。
DOI:
--
发表时间:
2010
期刊:
J Reprod Dev. 56(4)
影响因子:
--
作者:
[Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H.]
通讯作者:
Tamada H.
IFN-gamma markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
在狗肿瘤模型中,IFN-γ与瘤内树突状细胞疫苗显着配合。
DOI:
--
发表时间:
2010
期刊:
Cancer Res. 70(18)
影响因子:
--
作者:
[Mito K, Sugiura K, Ueda K, Hori T, Akazawa T, Yamate J, Nakagawa H, Hatoya S, Inaba M, Inoue N, Ikehara S, Inaba T.]
通讯作者:
Inaba T.
Effect of IL-12 on the canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4.
IL-12 对粒细胞-巨噬细胞 CSF 和 IL-4 诱导分化后的犬树突状细胞成熟的影响。
DOI:
--
发表时间:
2010
期刊:
Vet.Immunol.Immunopathol. 137(3-4)
影响因子:
--
作者:
[Sugiura K., Wijewardana V., Fujimoto M., Akazawa T., Yahata M., Mito K., Hatoya S., Inoue N., Inaba T.]
通讯作者:
Inaba T.
共 17 条
Culture of feline pluripotent stem cells and development of hematopoietic differentiation methods to aim for clinically applying for blood transfusion
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批准号:24780311
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$3.0万
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财政年份:2012
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负责人:HATOYA Shingo
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依托单位:
Development of system to establish canine embryonic stem cell line
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批准号:19880026
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项目类别:Grant-in-Aid for Young Scientists (Start-up)
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资助金额:$2.0万
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财政年份:2007
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负责人:HATOYA Shingo
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依托单位:
海外基金