Physiological role of SCN micro-circuit on circadian clock

SCN微电路对生物钟的生理作用

• 批准号: 21790073
• 负责人: YAMAGUCHI Yoshiaki
• 金额: $ 2.83万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790073/
• 关键词: 体内時計; 視交叉上核; グルタミン酸受容体; 時計遺伝子; サーカディアンリズム; AMPA受容体; Per; 概日リズム; アラトスタチン; 神経機能抑制; リズム同調; マウス; アデノ随伴ウイルス

The glutamatergic neurotransmission in the suprachiasmatic nucleus (SCN) plays a central role in the entrainment of the circadian rhythms to environmental light-dark cycles. Although the glutamatergic effect operating via NMDAR is well elucidated, much less is known about a role of AMPAR in circadian entrainment. Here I show that in vivo microinjection of AMPA in the SCN during the early subjective night phase-delays the behavioral rhythm. These data demonstrate that activation of AMPAR is capable of phase-shifting the circadian clock both in vivo and in vitro, and are consistent with the hypothesis that activation of AMPA receptors is a critical step in the transmission of photic information to the SCN.

视交叉上核（SCN）中的谷氨酸能神经传递在昼夜节律对环境光暗循环的影响中起着核心作用。虽然通过NMDAR操作的谷氨酸能效应已被很好地阐明，但对AMPAR在昼夜节律干扰中的作用知之甚少。在这里，我展示了在早期主观夜间阶段在SCN体内显微注射AMPA会延迟行为节律。这些数据表明，在体内和体外，AMPAR的激活都能够使生物钟相移，并且与AMPA受体的激活是光信息向SCN传递的关键步骤的假设一致。

项目成果

Circadian regulation of intracellular G-protein signalling mediates intercellular synchrony and rhythmicity in the suprachiasmatic nucleus.

• DOI: 10.1038/ncomms1316
• 发表时间: 2011
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Doi, Masao;Ishida, Atsushi;Miyake, Akiko;Sato, Miho;Komatsu, Rie;Yamazaki, Fumiyoshi;Kimura, Ikuo;Tsuchiya, Soken;Kori, Hiroshi;Seo, Kazuyuki;Yamaguchi, Yoshiaki;Matsuo, Masahiro;Fustin, Jean-Michel;Tanaka, Rina;Santo, Yasuko;Yamada, Hiroyuki;Takahashi, Yukari;Araki, Michihiro;Nakao, Kazuki;Aizawa, Shinichi;Kobayashi, Masaki;Obrietan, Karl;Tsujimoto, Gozoh;Okamura, Hitoshi
• 通讯作者: Okamura, Hitoshi

Hypertension due to loss of clock : novel insight from the molecular analysis of cry1/cry2-deleted mice

时钟丢失导致的高血压：cry1/cry2 缺失小鼠的分子分析的新见解

• DOI: 10.1007/s11906-011-0181-3
• 发表时间: 2011
• 期刊: Current hypertension reports
• 影响因子: 5.6
• 作者: Okamura H;Doi M;Yamaguchi Y;Fustin JM
• 通讯作者: Fustin JM

Molecular clocks in mouse skin

小鼠皮肤中的分子钟

• 发表时间: 2009
• 期刊: J Invest Dermatol 129
• 作者: Tanioka M;Yamada H;Doi M;Bando H;Yamaguchi Y;Nishigori C;Okamura H
• 通讯作者: Okamura H

季節変動と日内変動(体内時計とは? 体内時計について教えてください.Q&Aでわかる肥満と糖尿病9)

季节变化和昼夜变化（什么是生物钟？请告诉我生物钟。通过问答 9 解释肥胖和糖尿病）

• 发表时间: 2010
• 作者: 山口賀章;岡村均
• 通讯作者: 岡村均

Molecular clocks in mouse skin.

小鼠皮肤中的分子钟。

• 发表时间: 2009
• 期刊: Journal of Investigative Dermatology 129
• 作者: Tanioka M;Yamada H;Doi M;Bando H;Yamaguchi Y;Nishigori C;Okamura H.
• 通讯作者: Okamura H.