Production of secretory IgA against Shiga-like toxin B subunit using liposomes entrapping MHC-binding peptides

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B subunit of Shiga-like toxin (Stx1B) has been demonstrated to be a poor immunogenicity in mice. To augments the immunogenicity of Stx1B, Stx1B and major histocompatibility complex class II bind peptides, including T cell epitopes were liposomalized. Mice were given Stx1B-liposomes via nasal cavity. As a result, lipsomalization of Stx1B efficiently augmented the immunogenicity of Stx1B in the mucosal immune systems. Moreover, secretory IgA was obtained by incubation of dimeric IgA and recombinant secretory component, or by transcytosis of the dimeric IgA through epithelial cells expressing poly-Ig receptor in vitro.