The functional analysis of autism related and BDNF secretion regulated protein CAPS2

脑源性神经营养因子(BDNF)在神经发生、神经元存活和发育以及突触发育和可塑性中发挥重要作用。然而，BDNF的分泌机制在很大程度上还不清楚。最近发现的钙依赖分泌激活蛋白2(CAPS2)是一种调节脑源性神经营养因子分泌的蛋白质。在本研究中，我们利用延时BDNF分泌成像技术证明了CAPS2不仅加速了BDNF的分泌动力学，而且还增加了BDNF的量子释放。此外，CAPS2调节的BDNF分泌在GABA能神经元间网络的发育中起着关键作用。GABA能神经元间损伤影响突触可塑性和焦虑样行为。

Brain derived neurotrophic factor (BDNF) plays critical role in neurogenesis, neuronal survival and development and in synaptic development and plasticity. However, the mechanism of the BDNF secretion is largely unknown. Recently, Ca^<2+> dependent activator protein for secretion 2 (CAPS2) has been discovered as a protein which regulates BDNF secretion. In the present study, we demonstrated that CAPS2 not only accelerates BDNF secretion kinetics but also increases BDNF quantal release by using time-lapse BDNF secretion imaging technique. In addition, CAPS2 regulated BDNF secretion plays a critical role in development of GABAergic interneuron network. The GABAergic interneuron impairments affect synaptic plasticity and anxiety-like behavior.