The functional analysis of autism related and BDNF secretion regulated protein CAPS2

自闭症相关及BDNF分泌调节蛋白CAPS2的功能分析

• 批准号: 21790219
• 负责人: SHINODA Yo
• 金额: $ 2.08万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790219/
• 关键词: 生理学; 精神科学; 脳・神経; BDNFCA; PS2; 分泌; CAPS2; BDNF; GABA; Interneuron; interneuron; anxiety; secretion

Brain derived neurotrophic factor (BDNF) plays critical role in neurogenesis, neuronal survival and development and in synaptic development and plasticity. However, the mechanism of the BDNF secretion is largely unknown. Recently, Ca^<2+> dependent activator protein for secretion 2 (CAPS2) has been discovered as a protein which regulates BDNF secretion. In the present study, we demonstrated that CAPS2 not only accelerates BDNF secretion kinetics but also increases BDNF quantal release by using time-lapse BDNF secretion imaging technique. In addition, CAPS2 regulated BDNF secretion plays a critical role in development of GABAergic interneuron network. The GABAergic interneuron impairments affect synaptic plasticity and anxiety-like behavior.

脑源性神经营养因子 (BDNF) 在神经发生、神经元存活和发育以及突触发育和可塑性中发挥着关键作用。然而，BDNF 分泌的机制在很大程度上尚不清楚。最近，已发现Ca 2+ 依赖性分泌激活蛋白2(CAPS2)作为调节BDNF分泌的蛋白质。在本研究中，我们通过使用延时 BDNF 分泌成像技术证明 CAPS2 不仅加速 BDNF 分泌动力学，而且还增加 BDNF 量子释放。此外，CAPS2调节的BDNF分泌在GABA能中间神经元网络的发育中起着关键作用。 GABA 能中间神经元损伤会影响突触可塑性和焦虑样行为。

项目成果

Secretory vesicle-related gene CAPS2 knock-out mice exhibit the reduced number of hippocampal GABAergic interneuron and the impairments in synaptic plasticity and behavior.

分泌囊泡相关基因 CAPS2 敲除小鼠表现出海马 GABA 能中间神经元数量减少以及突触可塑性和行为受损。

• 发表时间: 2009
• 作者: Yo Shinoda;Tetsushi Sadakata;Asako Furuya;Ritsuko Katoh-Semba;Teiichi Furuichi.
• 通讯作者: Teiichi Furuichi.

Enhancement of BDNF secretion kinetics by the secretory vesicle-associated protein CAPS2 : reduced BDNF levels, impaired development of hippocampal GABAergic neurons and increased anxiety-like behavior in CAPS2 KO mice

分泌囊泡相关蛋白 CAPS2 增强 BDNF 分泌动力学：CAPS2 KO 小鼠 BDNF 水平降低，海马 GABA 能神经元发育受损，焦虑样行为增加

• 发表时间: 2010
• 作者: Yo Shinoda;Tetsushi Sadakata;Asako Furuya;Ritsuko Katoh-Semba;Teiichi Furuichi.;Yo Shinoda;Yo Shinoda
• 通讯作者: Yo Shinoda

Persistent synapse loss induced by repetitive LTD in developing rat hippocampal neurons.

• DOI: 10.1371/journal.pone.0010390
• 发表时间: 2010-04-28
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Shinoda Y;Tanaka T;Tominaga-Yoshino K;Ogura A
• 通讯作者: Ogura A

Secretion-associated protein CAPS2 KO mice show impairments in BDNF expression and synapse function in hippocampus and exhibit anxiety-like and/or depression-like behaviors.

分泌相关蛋白 CAPS2 KO 小鼠海马 BDNF 表达和突触功能受损，并表现出焦虑样和/或抑郁样行为。

• 发表时间: 2009
• 作者: Yo Shinoda;Tetsushi Sadakata;Asako Furuya;Ritsuko Katoh-Semba;Teiichi Furuichi.
• 通讯作者: Teiichi Furuichi.

Expression of the IP3R1 Promoter-Driven nls-LacZ Transgene in Purkinje Cell Parasagittal Arrays of Developing Mouse Cerebellum

• DOI: 10.1002/jnr.22451
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF NEUROSCIENCE RESEARCH
• 影响因子: 4.2
• 作者: Furutama, Daisuke;Morita, Noriyuki;Furuichi, Teiichi
• 通讯作者: Furuichi, Teiichi