Analysis of apolipoprotein CIII function with transgenic rabbit

转基因兔载脂蛋白CIII功能分析

• 批准号: 21790322
• 负责人: NISHIJIMA Kazutoshi
• 金额: $ 1.66万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790322/
• 关键词: 動脈硬化; 遺伝子組換え; 病態モデル; ウサギ

We attempted to create a transgenic rabbit as a tool to analyze the function of apolipoprotein(apo) CIII on lipid metabolism. 2727 ova were microinjected with human apoCIII expression DNA construct. 2276 embryos were transferred into 87 recipient rabbits, and 24 offspring were born. As a result of PCR analysis, no integration of human apoCIII gene was recognized in the offspring.

我们试图建立一个转基因兔作为一种工具，分析载脂蛋白（apo）CIII对脂质代谢的功能。用人apoCIII表达DNA构建体显微注射2727个卵。将2276枚胚胎移植到87只受体兔体内，产仔24只。作为PCR分析的结果，在后代中没有识别到人apoCIII基因的整合。