Comprehensive analysis base on the interaction between follicular lymphoma cells and follicular dendritic cells

为了阐明滤泡性淋巴瘤发生的机制，我们采用激光显微切割和鸟枪蛋白质组学技术进行了全面的蛋白质检测分析。我们检测了近2000种蛋白质，主要集中在GRHPR和PACAP，这两种蛋白质分别在滤泡性淋巴瘤和非肿瘤性滤泡中频繁检测。这些基因在转录水平上过表达，并且在人类组织学样本中证实了每个蛋白质的表达。本研究提示这些基因可能是滤泡性淋巴瘤发生的候选标志物，需要进一步的分子生物学和功能分析。

To elucidate mechanism of lymphomagenesis of follicular lymphoma, we examined comprehensive protein detection analysis using laser microdissection and shotgun proteomics. We detected approximately two thousand kinds of proteins, focused on GRHPR and PACAP, which detected frequently in follicular lymphoma, and non-neoplastic follicle respectively. These genes were over-expressed in transcriptional level and each protein expressions are affirmed in human histology sample. This study suggested these genes are the candidate marker in follicular lymphomagenesis, and further molecular biological and functional analyses are desirable.