Apicomplexa specific traffic pathway in protozoa

原生动物中顶端复合体特定的交通途径

• 批准号: 21790411
• 负责人: NAKANO Yumiko
• 金额: $ 2.75万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790411/
• 关键词: 原虫; small GTPase; メンブレントラフィック; Rab5; アピコンプレキサ; Plasmodium falciparu; 感染症; Plasmodium falciparum

One of the small GTPase, PfRab5a, was previously reported that involvement of endocytosis of erythrocyte cytosol. This work shows that another Rab5 isotype, Rab5b, possesses conserved motif with myristoilation site in N-terminus. Rab5b homologue is presented in Plasmodium and Toxopasma but not in Cryptosporidium, Paramecium and Tetrahymena in protozoa classified in Alveolata. Although expression of PfRab5b was low among three Rab5 isotypes, PfRab5b was expressed in erythrocytic stage. Expression of recombinant protein of PfRab5b was effective when an artificial gene appropriate for E. coli K12 codon was used.

PfRab 5a是一种小的GTbR，以前曾报道它参与红细胞胞浆的内吞作用。本工作表明Rab 5的另一个同种型Rab 5 b具有保守的基序，其N-端具有豆蔻酰化位点。Rab 5 b同源物存在于疟原虫和弓形虫中，但不存在于泡状纲原生动物中的隐孢子虫、草履虫和四膜虫中。虽然PfRab 5 b在三种Rab 5亚型中表达较低，但PfRab 5 b在红细胞期表达。用适合大肠杆菌表达的人工基因进行PfRab 5 b重组蛋白的表达。coliK 12密码子。

项目成果

Diversity and Significance of Vesicular Trafficking in Entamoeba histolytica.

溶组织内阿米巴囊泡贩运的多样性和意义。

• 发表时间: 2010
• 作者: Yumiko Saito-Nakano;Mami Okada;Gil Mallari Penuliar;Yuki Hanadate;Carol A.Gilchrist;Oswald Crasta;William A.Petri;Jr.;Zhangjun Fei;Nino Trapaidze;Tomoyoshi Nozaki.
• 通讯作者: Tomoyoshi Nozaki.

Marked Amplification and Diversification of Products of ras Genes from Rat Brain, Rab GTPases, in the Ciliates Tetrahymena thermophila and Paramecium tetraurelia

• DOI: 10.1111/j.1550-7408.2010.00503.x
• 发表时间: 2010-09
• 期刊: Journal of Eukaryotic Microbiology
• 影响因子: 2.2
• 作者: Y. Saito-Nakano;Tohru Nakahara;K. Nakano;T. Nozaki;O. Numata