Apicomplexa specific traffic pathway in protozoa

一个小的GTPase， PfRab5a，先前报道参与红细胞胞浆的内吞作用。这项工作表明Rab5的另一个同型Rab5b在n端具有肉芽化位点的保守基序。在肺泡门原虫中，隐孢子虫、草履虫和四膜虫中没有Rab5b同源物。虽然PfRab5b在三种Rab5亚型中表达较低，但PfRab5b在红细胞阶段表达。当使用大肠杆菌K12密码子的人工基因时，PfRab5b重组蛋白的表达是有效的。

One of the small GTPase, PfRab5a, was previously reported that involvement of endocytosis of erythrocyte cytosol. This work shows that another Rab5 isotype, Rab5b, possesses conserved motif with myristoilation site in N-terminus. Rab5b homologue is presented in Plasmodium and Toxopasma but not in Cryptosporidium, Paramecium and Tetrahymena in protozoa classified in Alveolata. Although expression of PfRab5b was low among three Rab5 isotypes, PfRab5b was expressed in erythrocytic stage. Expression of recombinant protein of PfRab5b was effective when an artificial gene appropriate for E. coli K12 codon was used.