Apicomplexa specific traffic pathway in protozoa

其中一个小的GTP酶PfRab5a，以前报道参与了红细胞胞浆的内吞作用。本工作表明，另一种Rab5亚型Rab5b在N-端有一个保守基序，其肉豆蔻环上有一个扩张点。Rab5b同源物存在于疟原虫和弓形虫中，而不存在于隐孢子虫、草履虫和四膜虫中。虽然PfRab5b在三种Rab5亚型中的表达较低，但在红细胞期有表达。使用与E.ColiK12密码子相适应的人工基因，可有效表达PfRab5b重组蛋白。

One of the small GTPase, PfRab5a, was previously reported that involvement of endocytosis of erythrocyte cytosol. This work shows that another Rab5 isotype, Rab5b, possesses conserved motif with myristoilation site in N-terminus. Rab5b homologue is presented in Plasmodium and Toxopasma but not in Cryptosporidium, Paramecium and Tetrahymena in protozoa classified in Alveolata. Although expression of PfRab5b was low among three Rab5 isotypes, PfRab5b was expressed in erythrocytic stage. Expression of recombinant protein of PfRab5b was effective when an artificial gene appropriate for E. coli K12 codon was used.