权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional analysis of effector of Vibrio parahaemolyticus TTSS2 on enterotoxicity.

副溶血性弧菌TTSS2肠毒性效应子的功能分析。

基本信息

批准号：
21790419
负责人：
KODAMA Toshio
金额：
$ 2.75万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790419/
关键词：
腸炎ビブリオ腸管毒性 3型分泌装置エフェクターエフェクー

项目摘要

Many Gram-negative bacterial pathogens use a specialized Type III secretion system (TTSS) to inject virulence factors, called effectors, directly into eukaryotic cells. T3SS2, a TTSS of Vibrio parahaemolyticus which is a pathogen causing food-borne disease worldwide, is indispensable for pathogenicity, and especially enterotoxicity, of this bacterium. However, which effector(s) is or are responsible for the T3SS2-dependent enterotoxicity has remained an open question. In this study, we identified a novel effector, VopE, as a major contributor to the enterotoxicity of Vibrio parahaemolyticus in the rabbit ileal loop test. The VopE protein consists of three domains, the N-terminal, the long repeat (LR), and the C-terminal domains. The LR domain contains three types of repeat units. We demonstrated that VopE directly binds to F-actin via a repeat unit (rep1) in the LR or the C-terminal domain, and that the enterotoxic activity generated by VopE correlated significantly with its F-actin binding activity. We further demonstrated that a TTSS2-related TTSS and a vopE homologous gene in a non-O1/non-O139 V. cholerae strain, which is pathogenic for humans, were also involved in the enterotoxicity of the strain. These results indicate that VopE is a novel F-actin-targeting effector and its ability to bind to F-actin is involved in the enterotoxic activity of TTSS2-possessing pathogens.

许多革兰氏阴性细菌病原体使用专门的III型分泌系统（TTSS）将称为效应子的毒力因子直接注入真核细胞。T3 SS 2是副溶血性弧菌（Vibrio parahaemolyticus）的一种TTSS，是该细菌致病性，尤其是肠毒性不可或缺的因子。然而，哪种效应物是T3 SS 2依赖性肠毒性的原因仍然是一个悬而未决的问题。在这项研究中，我们确定了一种新的效应，VopE，作为一个主要的贡献者的肠毒性副溶血性弧菌在兔回肠袢试验。VopE蛋白由三个结构域组成，N-末端、长重复序列（LR）和C-末端结构域。LR结构域包含三种类型的重复单元。我们证明了VopE通过LR或C-末端结构域中的重复单元（rep 1）直接结合F-肌动蛋白，并且VopE产生的肠毒性活性与其F-肌动蛋白结合活性显著相关。我们进一步证明，TTSS 2相关的TTSS和vopE同源基因在非O 1/非O 139霍乱弧菌菌株，这是人类的致病性，也参与了菌株的肠毒性。这些结果表明，VopE是一种新型的F-肌动蛋白靶向效应子，其与F-肌动蛋白结合的能力参与了TTSS 2的肠道毒性活性。