Analysis of qualitative and quantitative secretory regulation of Vibrio parahaemolyticusT3SS2

副溶血弧菌T3SS2的定性和定量分泌调节分析

• 批准号: 23790474
• 负责人: KODAMA Toshio
• 金额: $ 2.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790474/
• 关键词: 腸炎ビブリオ; 3型分泌装置; エフェクター; Vibrio parahaemolyticus; T3SS; effector; enterotoxicity

Type III secretion system 2 of Vibrio parahaemolyticus is essential for entrotoxicity. However, qualitative and quantitative regulation of T3SS2 is unclear. In this study, I identified two novel genes responsible for secretory regulation of T3SS2. The knock-out strains of these genes caused a dramatic change in a secretory pattern of T3SS2 secreted proteins, indicating that these genes are involved in qualitative and quantitative secretory regulation of Vibrio parahaemolyticus T3SS2.

副溶血性弧菌的III型分泌系统2对其毒性至关重要。然而，T3SS2的定性和定量调控尚不清楚。在这项研究中，我发现了两个负责T3SS2分泌调节的新基因。这些基因的敲除菌株导致T3SS2分泌蛋白的分泌模式发生了巨大变化，表明这些基因参与了副溶血性弧菌T3SS2的定性和定量分泌调控。

项目成果

Vibrio vulnificus hemolysin associates with detergent-resistant membranes, but this localization is not essential for its cytotoxicity

创伤弧菌溶血素与耐洗涤剂的膜相关，但这种定位对于其细胞毒性来说并不是必需的

• 发表时间: 2012
• 作者: 小椋義俊;林哲也;Takashige Kashimoto
• 通讯作者: Takashige Kashimoto

An novel F-actin-binding type III secretion effector is required for Vibrio parahaemolyticus-induced enterotoxicity

副溶血性弧菌诱导的肠毒性需要一种新型 F-肌动蛋白结合 III 型分泌效应器

• 发表时间: 2012
• 作者: Mima T;Schweizer HP;Xu ZQ;大岡唯祐,勢戸和子,河野喜美子,小林秀樹,江藤良樹,市原祥子,金子紀子,磯部順子,山口敬治,堀川和美,小椋義俊,林哲也;Hirotaka Hiyoshi
• 通讯作者: Hirotaka Hiyoshi

Haemolysin E- and enterohaemolysin-derived haemolytic activity of O55/O157 strains and other Escherichia coli lineages.

• DOI: 10.1099/mic.0.054775-0
• 发表时间: 2012-03
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: K. Murase;Tadasuke Ooka;Atsushi Iguchi;Yoshitoshi Ogura;K. Nakayama;M. Asadulghani;Md. Rakibul Islam;Hirotaka Hiyoshi;T. Kodama;L. Beutin;Tetsuya Hayashi

VopC, a Vibrio parahaemolyticus T3SS2 effector, mediates invasion and actin stress fiber formation

VopC 是副溶血弧菌 T3SS2 效应子，介导侵袭和肌动蛋白应力纤维形成

• 发表时间: 2013
• 作者: J.G.Mainil;et al;Ryu Okada
• 通讯作者: Ryu Okada

Relationship between localization and cytotoxicity of Vibrio vulnificus hemolysin

创伤弧菌溶血素定位与细胞毒性的关系

• 发表时间: 2012
• 作者: 小林泰良;小川道永;笹川千尋;柏本孝茂
• 通讯作者: 柏本孝茂