Analysis of the interaction between enteropathogenic Escherichia coli infection and mucosal immunity in vitro and in vivo

肠道致病性大肠杆菌感染与体内外黏膜免疫的相互作用分析

• 批准号: 21790428
• 负责人: NAGAI Takeshi
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790428/
• 关键词: 病原細菌; 免疫; 細菌感染; 腸管免疫; 微生物; 免疫学; 細菌

The aim of this study is to investigate the interaction between enteropathogenic E.coli (EPEC) and host mucosal immune response. Consequently, we found that Peyer's patch was important for bacterial colonization in C57BL/6 mouse but not Balb/c mouse. And then we performed the in vitro infection model using bonemarrow derived dendritic cells and epithelial cells. Consequently, type III secretion system of EPEC directly or indirectly regulated the expression of various genes on dendritic cells.

本研究的目的是探讨致病性大肠埃希菌(EPEC)与宿主黏膜免疫反应的相互作用。因此，我们发现Peyer‘s补丁对C57BL/6小鼠的细菌定植很重要，而对Balb/c小鼠的定植作用不重要。然后利用骨髓来源的树突状细胞和上皮细胞进行体外感染模型。因此，EPEC的III型分泌系统直接或间接调控树突状细胞上各种基因的表达。

项目成果

Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment

• DOI: 10.1038/nature07952
• 发表时间: 2009-05-28
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Kim, Minsoo;Ogawa, Michinaga;Sasakawa, Chihiro
• 通讯作者: Sasakawa, Chihiro

Listerial invasion protein internalin B promotes entry into ileal Peyer's patches in vivo

• DOI: 10.1111/j.1348-0421.2010.00292.x
• 发表时间: 2011-02-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Chiba, Sayuri;Nagai, Takeshi;Koyasu, Shigeo
• 通讯作者: Koyasu, Shigeo