Analysis of dengue virus quasispecies and their cell tropism.

众所周知，登革热病毒（DENV）以各种病毒种群的形式存在，即所谓的准种。本研究以DENV准种为研究对象，为DENV分析开辟了新的途径。DENV继发感染时易引起重症。我们分析了原发性和继发性感染的DENV序列，发现原发性感染的DENV在DENV基因组中具有广泛的变异，而继发性感染的DENV则具有同质性。这表明继发感染的DENV在继发感染中得到了有效的选择。此外，我们还开发了一种有效的DENV准种分析方案，并观察到病毒群体转换为一个群体。

Dengue virus (DENV) is known to exist as various virus population, so-called quasispecies. In this study, we focused on the DENV quasispecies to take a new approach to analyze DENV. DENV easily cause severe illness when patients are secondary infected with DENV. We analyzed the sequences of DENV derived from primary and secondary infection, and then found that the DENV from primary infections carried broad range of variation in DENV genomes, contrary DENV from secondary infection were homogenous. This suggests that DENV from secondary infection effectively selected in secondary infections. Furthermore, we developed an effective protocol for the analysis of DENV quasispecies and observed the virus population was switch to one population.