Control of tumor formation after transplantation in regenerative therapy with genetic engineering methods

基因工程方法再生治疗中移植后肿瘤形成的控制

• 批准号: 21790528
• 负责人: TAHARA Tsuyoshi
• 金额: $ 2.75万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790528/
• 关键词: 再生医療; がん; HSV-TK; 癌

Gene therapy and cell transplantation therapy is expected to be a breakthrough for treating neurodegenerative disorders, such as Parkinson's and Alzheimer's disease. In this study, I could isolate the tumor- or non-differentiated cell-specific promoter region from Oct3/4 gene promoter and make the GFP expression vector, which works only in non-differentiated cell, such as mouse embryonic EB5 cell but not mouse fibroblast Balb/3T3b cells.

基因疗法和细胞移植疗法有望成为治疗帕金森氏症和阿尔茨海默病等神经退行性疾病的突破口。在这项研究中，我可以从Oct3/4基因启动子中分离出肿瘤或未分化细胞特异性启动子区域，并制成GFP表达载体，该载体仅适用于未分化细胞，如小鼠胚胎EB5细胞，而不适用于小鼠成纤维细胞Balb/3T3b细胞。