Development of "microRNA-regulating chemoprevention"

MIR-34a是一种肿瘤抑制因子microRNA。我搜索了调节miR-34a表达的食物成分。流式细胞仪分析的结果是，利用RB灭活的癌细胞，发现了几种诱导G1期停滞的材料。实时荧光定量聚合酶链式反应(Real-Time PCR)确定了诱导候选材料miR-34a表达的黄酮类化合物。此外，通过Western印迹分析证实miR-34a的靶蛋白减少，这些结果表明食物成分可以在体内控制microRNA的表达。研究黄酮调控的miR-34a有可能实现“microRNA调控的化学预防”

miR-34a is a tumor suppressor microRNA. I searched for the food components that regulated miR-34a expression. As a result from flow cytometry analysis, several kinds of materials inducing G1 arrest were found, using RB inactivated cancer cells.Real-time PCR specified flavone that induced miR-34a expression from candidate materials. In addition, decrease in target proteins of miR-34a was confirmed by Western blot analysis.These results suggested that food component could control expression of microRNA in vivo. The research of miR-34a regulated by flavone might realize "microRNA-regulating chemoprevention"