Evaluation the carcinogenicity of nanomaterials from the mechanochemical treatment of asbestos.

对石棉进行机械化学处理有望使石棉分解并转化为新型纳米材料。为了评估温石棉机械化学处理产生的纳米材料的致癌性，我们将温石棉（CH）和纳米材料（CH- m）单次腹腔注射给大鼠。在1年的研究中，间皮瘤的发病率是通过组织病理学来确定的。此外，我们还测定了尿中8-羟基-2'-脱氧鸟苷（8-OHdG）的水平，这是一种氧化DNA损伤的生物标志物，以及血清中N-ERC/间皮瘤的生物标志物。虽然在60%的ch治疗大鼠中检测到间皮瘤，但在CH-M治疗的大鼠中未观察到间皮瘤。在CH组，180天后8-OHdG和N-ERC/间皮素水平升高。与CH组相比，CH- m组8-OHdG和N-ERC/间皮素水平显著降低。我们的研究结果共同表明，CH的机械化学处理降低了氧化应激和致癌潜力。

The mechanochemical treatment of asbestos is expected to decompose asbestos and transform into new nanomaterials. To evaluate the carcinogenicity of nanomaterials produced by the mechanochemical treatment of chrysotile, we administered chrysotile (CH) and the nanomaterial (CH-M) to rats by a single intraperitoneal injection. The incidence of mesothelioma was determined histopathologically during the 1-year study. In addition, we determined urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker for oxidative DNA damage, and serum levels of N-ERC/mesothelin, a biomarker for mesothelioma. While mesothelioma was detected in 60% of CH-treated rats, it was not observed in rats treated with CH-M. In the CH group, 8-OHdG and N-ERC/mesothelin levels were elevated after day 180. Levels of 8-OHdG and N-ERC/mesothelin were significantly lower in the CH-M group compared to the CH group. Our results collectively suggested that mechanochemical treatment of CH reduced oxidative stress and carcinogenic potential.