Evaluation the carcinogenicity of nanomaterials from the mechanochemical treatment of asbestos.

石棉的机械力化学处理有望分解石棉并转化为新的纳米材料。为了评价机械力化学处理温石棉产生的纳米材料的致癌性，我们用温石棉(CH)和纳米材料(CH-M)一次腹腔注射给大鼠。在为期一年的研究中，组织病理学检查了间皮瘤的发生率。此外，我们还测定了尿8-羟基-2‘-脱氧鸟苷(8-OHdG)和血清N-ERC/Mesothelin的水平，8-羟基-2’-脱氧鸟苷(8-OHdG)是DNA氧化损伤的生物标志物，N-ERC/Mesothelin是间皮瘤的生物标志物。在CH-M处理的大鼠中，60%的大鼠发现间皮瘤，而CH-M处理的大鼠则没有。在CH组，8-OHdG和N-ERC/Mesothelin水平在180天后升高。CH-M组的8-OHdG和N-ERC/Mesothelin水平明显低于CH组。我们的结果表明，机械力化学处理的CH降低了氧化应激和致癌潜力。

The mechanochemical treatment of asbestos is expected to decompose asbestos and transform into new nanomaterials. To evaluate the carcinogenicity of nanomaterials produced by the mechanochemical treatment of chrysotile, we administered chrysotile (CH) and the nanomaterial (CH-M) to rats by a single intraperitoneal injection. The incidence of mesothelioma was determined histopathologically during the 1-year study. In addition, we determined urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker for oxidative DNA damage, and serum levels of N-ERC/mesothelin, a biomarker for mesothelioma. While mesothelioma was detected in 60% of CH-treated rats, it was not observed in rats treated with CH-M. In the CH group, 8-OHdG and N-ERC/mesothelin levels were elevated after day 180. Levels of 8-OHdG and N-ERC/mesothelin were significantly lower in the CH-M group compared to the CH group. Our results collectively suggested that mechanochemical treatment of CH reduced oxidative stress and carcinogenic potential.