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Evaluation of biological effects of thermally decomposed asbestos

热分解石棉的生物效应评价

基本信息

批准号：
18590576
负责人：
TAKATA Ayako
金额：
$ 2.53万
依托单位：
St.Marianna University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590576/
关键词：
asbestos waste recycling carcinogenicity oxidative DNA damage forsterite アズベスト

项目摘要

The conversion of chrysotile (CH) to forsterite (FO) by thermal treatment is considered to be an acceptable technique for detoxifying chrysotile. In this study, the biological effects of CH and an FO that is synthesized by heating CH at 1000℃ were compared with respect oxidative DNA damage and lung injury.Following the administration of a single 1-mg intratracheal dose of CH or FO, we evaluated histopathological changes in the lung tissue, the lung concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) by HPLC-electrochemical detection (ECD) method, and the expression of 8-OHdG by immunostaining for 540 days. For the control group, we administered a single intratracheal dose of sterile saline.In the early period following the intratracheal administration of CH, expression of 8-OHdG was observed in the bronchiolar and alveolar epithelial cells as well as in inflammatory cells and granulomas ; at the same time, an increase in the lung concentration of 8-OHdG was observed. As fibrosis progressed, expression of 8-OHdG became more apparent in the airway epithelial and inflammatory cells surrounding the fibrotic lesions ; even at 540 days post-administration, the expression of 8-OHdG was significantly higher than that of the control group. On the other hand, compared with the CH group, the acute lung inflammation observed in the FO group was less apparent and exhibited no progressive fibrosing lesions. The expression of 8-OHdG was transient and weak in the bronchiolar epithelial cells as well as in the inflammatory cells, which was consistent with the low concentration of 8-OHdG in the lungs. This study demonstrates that synthetic FO causes significantly less inflammation and oxidative DNA damage in the lungs than CH.

温石棉（CH）转化为锻造石（FO）的热处理被认为是一种可接受的技术解毒温石棉。本研究比较了CH和CH在1000℃加热合成的FO在DNA氧化损伤和肺损伤方面的生物学效应。在气管内给药1 mg CH或FO后，我们观察了肺组织的组织病理学变化，采用hplc -电化学检测（ECD）方法观察了8-羟基-2′-脱氧鸟苷（8-OHdG）的肺浓度，并通过免疫染色观察了540天8-OHdG的表达。对于对照组，我们给予单次气管内无菌生理盐水。气管内给药后早期，细支气管和肺泡上皮细胞以及炎症细胞和肉芽肿中观察到8-OHdG的表达；同时观察到8-OHdG肺浓度升高。随着纤维化的进展，8-OHdG在纤维化灶周围的气道上皮细胞和炎症细胞中的表达更加明显；即使在给药后540天，8-OHdG的表达也明显高于对照组。另一方面，与CH组相比，FO组观察到的急性肺部炎症不太明显，未出现进行性纤维化病变。8-OHdG在细支气管上皮细胞和炎症细胞中的表达是短暂的、微弱的，这与肺中8-OHdG的低浓度一致。本研究表明，合成鱼油在肺部引起的炎症和氧化性DNA损伤明显小于CH。