The role of Wnt-5a as the pathological marker of inflammatory bowel disease.

Wnt-5a作为炎症性肠病病理标志物的作用。

• 批准号: 21790688
• 负责人: UCHIYAMA Kazuhiko
• 金额: $ 0.92万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790688/
• 关键词: Wnt-5a; 潰瘍性大腸炎; クローン病; myofibroblast; Hsp25; SERPINB1; Myofibroblast; FGF15

Colonic myofibroblast cells(VUPF) conditioned medium inhibited butyrate-induced Hsp25 expression and promoted butyrate induced cell proliferation. Mass spectroscopy of the active CM fraction implicated members of the Wnt-5a. Wnt5a expression was silenced in vivo by intramural injection with Wnt5a siRNA. At the site of injection, Hsp25 expression was increased along the entire length of the crypt in contrast to being only expressed by surface colonocytes in mice injected with scrambled siRNA. These findings were associated with decreased pericrypt MF Wnt-5a expression and decreased epithelial Ki-67 staining. When colonic crypt Hsp25 expression was increased through luminal lentiviral infection, Ki-67 staining was nearly absent. Wnt-5a mRNA expression of colonic mucosa from ulcerative colitis patients were significantly increased compated to normal control and remission stage of ulcerative colitis.

结肠肌成纤维细胞（VUPF）条件培养基抑制丁酸诱导的Hsp 25表达，促进丁酸诱导的细胞增殖。活性CM级分的质谱分析涉及Wnt-5a的成员。通过壁内注射Wnt 5a siRNA在体内沉默Wnt 5a表达。在注射部位，Hsp 25的表达沿着整个隐窝长度增加，与之相反，在注射了乱序siRNA的小鼠中，Hsp 25仅由表面结肠细胞表达。这些发现与微隐周MF Wnt-5a表达减少和上皮Ki-67染色减少相关。当结肠隐窝Hsp 25表达增加，通过管腔慢病毒感染，Ki-67染色几乎不存在。溃疡性结肠炎患者结肠黏膜中Wnt-5a mRNA的表达较正常对照组和缓解期明显增高。

项目成果

FGF19 protects colonic epithelial cells against hydrogen peroxide

FGF19 保护结肠上皮细胞免受过氧化氢的影响

• DOI: 10.1159/000321809
• 发表时间: 2011
• 期刊: Digestion
• 影响因子: 3.2
• 作者: Uchiyama K;Naito Y;Takagi T;Mizushima K;Hayashi N;Handa O;Ishikawa T;Yagi N;Kokura S;Yoshikawa T
• 通讯作者: Yoshikawa T

Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis

• DOI: 10.1152/ajpgi.00292.2011
• 发表时间: 2012-05-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
• 影响因子: 4.5
• 作者: Uchiyama, Kazuhiko;Naito, Yuji;Yoshikawa, Toshikazu
• 通讯作者: Yoshikawa, Toshikazu

Myofibroblast-derived FGF15/19 protects colonic mucosa and enhances mucosal repair in IBD

肌成纤维细胞衍生的 FGF15/19 保护 IBD 结肠粘膜并增强粘膜修复

• 发表时间: 2010
• 作者: Uchiyama K;Naito Y;et al.
• 通讯作者: et al.

Carbon monoxide (CO) enhance colonic epithelial restitution via FGF15 derived from colonic myofibroblast

一氧化碳 (CO) 通过源自结肠肌成纤维细胞的 FGF15 增强结肠上皮恢复

• 发表时间: 2010
• 期刊: Biochemical and Biophysical Research Communications 391(1)
• 作者: Uchiyama K;Naito Y;Takagi T;et al.;Kazuhiko Uchiyama
• 通讯作者: Kazuhiko Uchiyama

大腸上皮細胞のHeat Shock Protein(Hsp) 25発現抑制の意義に関する検討.大腸Myofibroblast由来Wnt-5aの役割

结肠肌成纤维细胞来源的 Wnt-5a 抑制结肠上皮细胞热休克蛋白 (Hsp) 25 表达的意义研究。

• 发表时间: 2009
• 作者: 内山和彦;内藤裕二他
• 通讯作者: 内藤裕二他