Isolation and characterization of a human monoclonal antibody against M2 of influenza A virus for reduction of avian influenza

抗甲型流感病毒 M2 的人单克隆抗体的分离和表征，用于减少禽流感

• 批准号: 21790767
• 负责人: OZAWA Tatsuhiko
• 金额: $ 2.75万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790767/
• 关键词: インフルエンザ; M2; 抗体医薬; インフルエザ

In this study, we applied ISAAC method to detect influenza virus-specific B-cells in peripheral blood from healthy volunteers who had been immunized with influenza HA vaccine to generate human influenza monoclonal antibodies. We obtained many human monoclonal antibodies reacting influenza viruses, and characterized their functions. One of them (Ab1-10) specifically bound external domain of the matrix protein 2 (M2e). We demonstrate that Ab1-10 reacted not only to seasonal influenza A viruses, but also to pandemic (H1N1) 2009 virus (2009 H1N1) and highly pathogenic avian influenza A virus. More importantly, it inhibited 2009 H1N1 viral replication in vitro, suggesting that it could be a potent candidate for medical application.

本研究应用ISAAC方法检测了流感HA疫苗免疫健康志愿者外周血中流感病毒特异性B细胞，以制备人流感单克隆抗体。我们获得了许多与流感病毒反应的人源单克隆抗体，并对其功能进行了鉴定。其中一株（Ab 1 -10）特异性结合基质蛋白2（M2 e）的外部结构域。我们证明了Ab 1 -10不仅对季节性甲型流感病毒有反应，而且对2009年H1N1大流行病毒（2009 H1N1）和高致病性禽流感病毒也有反应。更重要的是，它在体外抑制了2009年H1N1病毒的复制，这表明它可能是一个潜在的医学应用候选者。

项目成果

Characterization of human anti-influenza M2 antibody obtained from peripheral blood of vaccinated volunteer

从接种疫苗的志愿者外周血中获得的人抗流感 M2 抗体的表征

• 发表时间: 2011
• 作者: A.Muraguchi;et.al
• 通讯作者: et.al

The novel method to detect single cytokine-secreting T-cell using a cell-microarray chip

使用细胞微阵列芯片检测单个细胞因子分泌 T 细胞的新方法

• 发表时间: 2010
• 作者: K.Tajiri;et.al
• 通讯作者: et.al

HBワクチンにより得られたモノクローナル抗体のHBV中和活性とエピトープ解析

乙型肝炎疫苗单克隆抗体的中和活性及表位分析

• 发表时间: 2009
• 作者: 田尻和人;小澤龍彦;金艾順;岸裕幸;村口篤
• 通讯作者: 村口篤

Generation of TRAIL-receptor 1-specific human monoclonal Ab by a combination of immunospot array assay on a chip and human Ab-producing mice

• DOI: 10.1002/eji.201040551
• 发表时间: 2010-12-01
• 期刊: EUROPEAN JOURNAL OF IMMUNOLOGY
• 影响因子: 5.4
• 作者: Jin, Aishun;Ozawa, Tatsuhiko;Muraguchi, Atsushi
• 通讯作者: Muraguchi, Atsushi

The novel method to detect single cytokine-secreting T-cell using a cell-microarray chip.

使用细胞微阵列芯片检测单个细胞因子分泌 T 细胞的新方法。

• 发表时间: 2010
• 作者: Tajiri K;Hatakeyama S;Kobayashi E;Ozawa T;Jin A;Kishi H;Sugiyama T;Muraguchi A.
• 通讯作者: Muraguchi A.