Development of rapid generation of antibodies with high affinity for improvement of detection limi

开发快速生成高亲和力抗体以改善检测限

• 批准号: 23790617
• 负责人: OZAWA Tatsuhiko
• 金额: $ 2.83万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790617/
• 关键词: 臨床検査医学; 高親和性抗体; ウサギモノクローナル抗体; ISAAC; 抗体医薬

Antigen-specific rabbit monoclonal antibodies （RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system for rapid RaMoAb generation and produce antigen-specific RaMoAbs with high affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.交付決定額

Antigen-specific rabbit monoclonal antibodies （RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system for rapid RaMoAb generation and produce antigen-specific RaMoAbs with high affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.

项目成果

単一細胞解析に基づく迅速な抗原特異的 T 細胞受容体クローニングシステム

基于单细胞分析的快速抗原特异性T细胞受体克隆系统

• 发表时间: 2011
• 作者: 小林栄治;浜名 洋;長井輝美;小澤龍彦;岸 裕幸;村口 篤
• 通讯作者: 村口 篤

Screening of Antigen-Specific Antibody-Secreting Cells

• DOI: 10.1007/978-981-10-4857-9_27-1
• 发表时间: 2021-10
• 期刊: Handbook of Single Cell Technologies
• 作者: M. N. Hsu;Z. Tay;W. Lin;Shih-Chung Wei

he binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβ antibody

通过与抗 Cβ 抗体交联，可溶性 TCR-Fc 融合蛋白的结合亲和力显着提高

• 发表时间: 2012
• 作者: 岸裕幸;小林栄治;水腰英四郎;小澤龍彦;金子周一;村口篤;Ozawa T
• 通讯作者: Ozawa T

シングル細胞チップと抗体医薬への応用

单细胞芯片及其在抗体药物中的应用

• 发表时间: 2011
• 作者: 小澤龍彦;岸裕幸;村口篤
• 通讯作者: 村口篤

The novel method to detect single antibody-secreting B-cells and single cytokine-secreting T -cells using a cell-microarray chip

使用细胞微阵列芯片检测单一抗体分泌 B 细胞和单一细胞因子分泌 T 细胞的新方法

• 发表时间: 2011
• 作者: Ozawa T .;Kobayashi E.;T ajiri K.;Jin A.;Kishi H.;Muraguchi A
• 通讯作者: Muraguchi A