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Development of rapid generation of antibodies with high affinity for improvement of detection limi

Development of rapid generation of antibodies with high affinity for improvement of detection limi
开发快速生成高亲和力抗体以改善检测限
批准号:
23790617
负责人:
OZAWA Tatsuhiko
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012

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中文摘要
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英文摘要
Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system for rapid RaMoAb generation and produce antigen-specific RaMoAbs with high affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.交付決定額
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会议论文
単一細胞解析に基づく迅速な抗原特異的 T 細胞受容体クローニングシステム
基于单细胞分析的快速抗原特异性T细胞受体克隆系统
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [小林栄治, 浜名 洋, 長井輝美, 小澤龍彦, 岸 裕幸, 村口 篤]
通讯作者: 村口 篤
DOI: 10.1007/978-981-10-4857-9_27-1
发表时间: 2021-10
期刊: Handbook of Single Cell Technologies
影响因子: --
作者: [M. N. Hsu;Z. Tay;W. Lin;Shih-Chung Wei]
通讯作者: M. N. Hsu;Z. Tay;W. Lin;Shih-Chung Wei
he binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβ antibody
通过与抗 Cβ 抗体交联,可溶性 TCR-Fc 融合蛋白的结合亲和力显着提高
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [岸裕幸, 小林栄治, 水腰英四郎, 小澤龍彦, 金子周一, 村口篤, Ozawa T]
通讯作者: Ozawa T
シングル細胞チップと抗体医薬への応用
单细胞芯片及其在抗体药物中的应用
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [小澤龍彦, 岸裕幸, 村口篤]
通讯作者: 村口篤
32
    Isolation and characterization of a human monoclonal antibody against M2 of influenza A virus for reduction of avian influenza
    • 批准号:
      21790767
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.75万
    • 财政年份:
      2009
    • 负责人:
      OZAWA Tatsuhiko
    • 依托单位:
    海外基金