Development of rapid generation of antibodies with high affinity for improvement of detection limi
Development of rapid generation of antibodies with high affinity for improvement of detection limi
批准号:
23790617
负责人:
OZAWA Tatsuhiko
金额:
$2.83万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system for rapid RaMoAb generation and produce antigen-specific RaMoAbs with high affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.交付決定額
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単一細胞解析に基づく迅速な抗原特異的 T 細胞受容体クローニングシステム
基于单细胞分析的快速抗原特异性T细胞受体克隆系统
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[小林栄治, 浜名 洋, 長井輝美, 小澤龍彦, 岸 裕幸, 村口 篤]
通讯作者:
村口 篤
DOI:
10.1007/978-981-10-4857-9_27-1
发表时间:
2021-10
期刊:
Handbook of Single Cell Technologies
影响因子:
--
作者:
[M. N. Hsu;Z. Tay;W. Lin;Shih-Chung Wei]
通讯作者:
M. N. Hsu;Z. Tay;W. Lin;Shih-Chung Wei
he binding affinity of a soluble TCR-Fc fusion protein is significantly improved by crosslinkage with an anti-Cβ antibody
通过与抗 Cβ 抗体交联,可溶性 TCR-Fc 融合蛋白的结合亲和力显着提高
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[岸裕幸, 小林栄治, 水腰英四郎, 小澤龍彦, 金子周一, 村口篤, Ozawa T]
通讯作者:
Ozawa T
シングル細胞チップと抗体医薬への応用
单细胞芯片及其在抗体药物中的应用
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[小澤龍彦, 岸裕幸, 村口篤]
通讯作者:
村口篤
The novel method to detect single antibody-secreting B-cells and single cytokine-secreting T -cells using a cell-microarray chip
使用细胞微阵列芯片检测单一抗体分泌 B 细胞和单一细胞因子分泌 T 细胞的新方法
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Ozawa T ., Kobayashi E., T ajiri K., Jin A., Kishi H., Muraguchi A]
通讯作者:
Muraguchi A
共 32 条
Isolation and characterization of a human monoclonal antibody against M2 of influenza A virus for reduction of avian influenza
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批准号:21790767
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.75万
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财政年份:2009
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负责人:OZAWA Tatsuhiko
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依托单位:
海外基金