Investigation of the iron sensoring mechanism involved in the regulation of hepcidin expression

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At first I demonstrated the characteristics of binding to transferrin and iron uptake of transferrin receptor 2 (TfR2) compared to 1 (TfR1). Then, I tried to establish the stable cell lines that overexpress TfR2 or HFE, and the stable cell lines in that TfR2 or HFE expression was suppressed by knockdown. The changes of the expression levels of those were determined by RT-PCR or western blot. Next, the effects of the changes of Tf-Fe_2 concentration in cell culture medium on hepcidin expressions in hepatoma-derived cells were determined, but I did not observe any significant change against my expectation. However, I focused on the possibility that non-hepatocyte might have function to sensor iron status, and found that monocyte-derived cell line might have that.