Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine

乙型肝炎疫苗诱导人单克隆抗体表位及中和能力分析

• 批准号: 21790958
• 负责人: TAJIRI Kazuto
• 金额: $ 2.75万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790958/
• 关键词: B型肝炎ウイルス; 中和抗体; Small-HBsAg; 抗体療法; 細胞マイクロアレイシステム

We analyzed using monoclonal antibodies for neutralizing HBV, which were obtained by cell-microarray system. Epitope analysis showed that extracellular loop lesion of HBs-Ag has the antigenesity and concerns HBV-neutralization. Combination of the antibodies showed the neutralizing capacity against various genotype of HBV including G145R which is known for the escape mutant. These findings imply that HBV-vaccination is useful for the prevention of HBV-infection with various genotype or mutant HBV. Furthermore, the possibilities of antibody treatment using monoclonal antibodies or their combination for HBV-infection may be suggested.

我们分析使用单克隆抗体中和HBV，这是通过细胞芯片系统获得。抗原表位分析表明，HBV-Ag细胞外环损伤具有抗原性，与HBV中和有关。抗体的组合显示出针对各种HBV基因型的中和能力，包括已知的逃逸突变体G145 R。这些结果表明，HBV疫苗接种是有用的，以预防HBV感染的各种基因型或变异的HBV。此外，使用单克隆抗体或其组合的抗体治疗HBV感染的可能性可能会被建议。

项目成果

Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array

使用基于芯片的免疫点阵列快速分离抗原特异性抗体分泌细胞

• DOI: 10.1038/nprot.2011.322
• 发表时间: 2011-05-01
• 期刊: NATURE PROTOCOLS
• 影响因子: 14.8
• 作者: Jin, Aishun;Ozawa, Tatsuhiko;Muraguchi, Atsushi
• 通讯作者: Muraguchi, Atsushi

Analysis of the epitope and neutralizing activity of monoclonal antibodies obtained from small-HBsAg va ccination.

小 HBsAg 疫苗接种获得的单克隆抗体的表位和中和活性分析。

• 发表时间: 2009
• 作者: 田尻和人;ら
• 通讯作者: ら

Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine

• DOI: 10.1016/j.antiviral.2010.04.006
• 发表时间: 2010-07-01
• 期刊: ANTIVIRAL RESEARCH
• 影响因子: 7.6
• 作者: Tajiri, Kazuto;Ozawa, Tatsuhiko;Muraguchi, Atsushi
• 通讯作者: Muraguchi, Atsushi

IN VIVO MODEL USING UPA/SCID MICE WITH HUMAN HEPATOCYTES TO STUDY CROSSGENOTYPE PROTECTION OF HBV AND A ROLE OF HBS ANTIGEN MUTATION IN IMMUNITY ESCAPE

使用具有人类肝细胞的 UPA/SCID 小鼠体内模型研究 HBV 的跨基因型保护以及 HBS 抗原突变在免疫逃逸中的作用

• 发表时间: 2010
• 作者: Sugauchi F;Tajiri K;et al.
• 通讯作者: et al.

Analysis of the epitope and neutralizing activity of monoclonal antibodies obtained from small-HBsAg vaccination

小 HBsAg 疫苗接种获得的单克隆抗体的表位和中和活性分析

• 发表时间: 2009
• 作者: 田尻和人;ら
• 通讯作者: ら