Analysis of the epitope and neutralizing capacity of human monoclonal antibodies induced by hepatitis B vaccine

我们分析使用单克隆抗体中和HBV，这是通过细胞芯片系统获得。抗原表位分析表明，HBV-Ag细胞外环损伤具有抗原性，与HBV中和有关。抗体的组合显示出针对各种HBV基因型的中和能力，包括已知的逃逸突变体G145 R。这些结果表明，HBV疫苗接种是有用的，以预防HBV感染的各种基因型或变异的HBV。此外，使用单克隆抗体或其组合的抗体治疗HBV感染的可能性可能会被建议。

We analyzed using monoclonal antibodies for neutralizing HBV, which were obtained by cell-microarray system. Epitope analysis showed that extracellular loop lesion of HBs-Ag has the antigenesity and concerns HBV-neutralization. Combination of the antibodies showed the neutralizing capacity against various genotype of HBV including G145R which is known for the escape mutant. These findings imply that HBV-vaccination is useful for the prevention of HBV-infection with various genotype or mutant HBV. Furthermore, the possibilities of antibody treatment using monoclonal antibodies or their combination for HBV-infection may be suggested.