Regulation of perinatal respiration-related activity and Cl- homeostasis : searching for the factors of sudden infant death syndrome in the brain

围产期呼吸相关活动和 Cl-稳态的调节：寻找大脑中婴儿猝死综合征的因素

• 批准号: 21791046
• 负责人: OKABE Akihito
• 金额: $ 2.75万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791046/
• 关键词: 乳幼児突然死症候群; KCC2; 細胞内Cl^-濃度; GABA_A受容体; 舌下神経核; 細胞内C1^-濃度; グリシン受容体

Cation-chloride cotransporters are considered to play critical roles in the regulation of intracellular chloride ion concentration ([Cl^-]_i), and hence in the control of neuronal function. First, I tested the immunoreactivity of KCC2 using KCC2 antibody in developmental mouse medulla. Immunoreactivity of KCC2 in hypoglossal nucleus at P0 was higher than that at P7, while that of vagus nerve nucleus did not change during same period. Since developmental changes in expression pattern of KCC2 associated with respiration-related rhythmic activity (RRA) in medulla are not yet well understood, I recorded RRA extracellulary from respiration-related neurons in medullary slice preparation obtained from mice of P0-7. Under these conditions, perfusion of 100μM GABA-containing ACSF transiently increased the frequency of RRA. The KCC2 inhibitor furosemide also increased the frequency of RRA. Thus Cl^- homeostasis likely regulates the activity of motor output in early postnatal stage.

阳离子 - 氯化物共转运蛋白被认为在调节细胞内氯离子浓度（[Cl^ - ] _ I）中起关键作用，因此在神经元功能的控制中起着关键作用。首先，我在发育的小鼠髓质中使用KCC2抗体测试了KCC2的免疫反应性。 KCC2在P0处的降低核中KCC2的免疫反应性高于P7的免疫反应性，而迷走神经核的免疫反应在同一时期没有变化。由于在髓质中与呼吸相关的节奏活性（RRA）相关的KCC2表达模式的发育变化尚未得到充分了解，因此我记录了从P0-7小鼠获得的髓样制剂中的RRA外胞外神经元中的RRA细胞外。在这些条件下，富含100μmGABA的ACSF的灌注会瞬时增加RRA的频率。 KCC2抑制剂速尿还增加了RRA的频率。这种Cl^ - 稳态可能调节产后早期的运动输出活性。

项目成果

PECULIAR ACCUMULATION OF GABAergic AND GLUTAMATERGIC NEURONS AT THE EARLY STAGE OF FREEZE LESION-INDUCED MICROGYRUS IN MICE

小鼠冰冻损伤引起的小回早期伽马能和谷氨酸神经元的特殊积累

• 发表时间: 2009
• 作者: Wang T;Kumada T;Morishima T;Okabe A;Yanagawa Y;Fukuda A.
• 通讯作者: Fukuda A.

Correlated expression of the neuron-specific potassium chloride cotransporter 2 (KCC2) in the neonatal cerebellum and inferior olive.

神经元特异性氯化钾协同转运蛋白 2 (KCC2) 在新生儿小脑和下橄榄中的相关表达。

• 发表时间: 2010
• 作者: Arata A;Okabe A;Takayama C;Fukuda A;Ito M.
• 通讯作者: Ito M.

PECULIAR ACCUMULATION OF GABAergic AND GLUTAMATERGIC NEURONS AT THE EARLY STAGE OF FREEZE LESION-INDUCED MICROGYRUS IN MICE.

小鼠冰冻损伤诱导小回早期伽马能和谷氨酸神经元的特殊积累。

• 发表时间: 2009
• 作者: Wang T;Kumada T;Morishima T;Okabe A;Yanagawa Y;Fukuda A.
• 通讯作者: Fukuda A.

Ontogeny of Cl^- homeostasis in mouse hypoglossal nucleus

小鼠舌下核 Cl^- 稳态的个体发育

• 发表时间: 2009
• 作者: Okabe A;Arata A;Oku Y;Takayama C;Fukuda A.
• 通讯作者: Fukuda A.

Function of ligand-gated chloride channels in the newborn rodent cerebral cortex

配体门控氯离子通道在新生啮齿动物大脑皮层中的功能

• 发表时间: 2009
• 作者: Luhmann HJ;Kilb W;Hanganu-Opatz IL;Okabe A;Sava BA;Shimizu-Okabe C;Fukuda A.
• 通讯作者: Fukuda A.