Rapid diagnosis for tiny sentinel node metastasis in malignant melanoma using RT-LAMP method

恶性黑色素瘤对化疗和放疗普遍耐药，是预后最差的恶性肿瘤之一。本研究旨在建立恶性黑色素瘤前哨淋巴结微小转移的快速诊断方法。因此，我们对大约30例黑色素瘤患者进行了前哨淋巴结活检，总共有50例。RT-PCR诊断与病理诊断符合率为82%，不符合率为18%。这一结果进一步证实了RT-PCR诊断恶性黑色素瘤微小前哨淋巴结转移是非常有用的方法。此外，我们还对RT-LAMP方法的可行性进行了验证，结果表明RT-LAMP和RT-PCR方法在检测恶性黑色素瘤微小转移灶方面具有相同的能力。我们认为，我们需要进一步的实验，以改善条件的RT-LAMP方法。

Malignant melanoma is generally resistant for chemotherapy and radiation therapy, and one of malignant tumors with the worst prognosis. The purpose of this study is to establish rapid diagnosis for tiny sentinel node metastasis in malignant melanoma. As a result, we have performed sentinel node biopsies about approximate 30 cases of melanoma patients, resulting in total of 50 cases. In 82% of cases, RT-PCR diagnosis is correspond to pathological diagnosis while RT-PCR is different from pathological diagnosis in 18%. This result further confirms that RT-PCR diagnosis for tiny sentinel node metastasis in malignant melanoma is very useful method. In addition, we examined feasibility of RT-LAMP method and showed that RT-LAMP and RT-PCR methods have almost the same ability to detect tiny metastasis of malignant melanoma. We think we need further experiments to improve conditions of RT-LAMP method.