Phenotypic analysis of keratinocyte-specific PBP null mice

角质形成细胞特异性 PBP 缺失小鼠的表型分析

• 批准号: 21791075
• 负责人: NAKAJIMA Takeshi
• 金额: $ 2.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791075/
• 关键词: 皮膚生理学

The interfollicular epidermis of keratinocyte-specific PBP null (PBP^<epi-/->) mice was acanthotic and the hair cycle of PBP^<epi-/-> mice was spontaneously repeated until about 6 months of age, which does not occur in wild-type mice. Consistently, immunohistochemical analysis showed keratinocytes in the epidermis of PBP^<epi-/-> were more proliferative. Flow cytometric analysis revealed that the numbers of hair follicle bulge stem cells were reduced in PBP^<epi-/-> mice from a few months after birth. From these results, it is suggested that PBP plays critical roles in the quiescence of hair follicle stem cells and that the follicular stem cells of PBP^<epi-/-> mice are depleted with age after continuously supplying excessive keratinocytes for the abnormally frequent repeat of hair cycling and the hyperproliferative interfollicular epidermis.

角化细胞特异性PBP缺失（PBP^<epi-/->）小鼠的毛囊间表皮是棘层的，并且PBP^<epi-/->小鼠的毛发周期自发地重复直到约6个月大，这在野生型小鼠中不发生。免疫组化结果显示PBP^<epi-/->表皮角质形成细胞增生明显。流式细胞术分析显示，毛囊隆突干细胞的数量在PBP^<epi-/->小鼠中从出生后几个月开始减少。从这些结果可以看出，PBP在毛囊干细胞的静止中起关键作用，并且PBP +<epi-/->小鼠的毛囊干细胞在持续供应过量的角质形成细胞用于毛发周期的异常频繁重复和过度增殖的毛囊间表皮之后随着年龄的增长而耗尽。

项目成果

In vitro and in vivo evidence of pathogenic roles of Hic-5/ ARA55 in keloids through Smad pathway and profibrotic transcription

Hic-5/ARA55 通过 Smad 通路和促纤维化转录在疤痕疙瘩中致病作用的体外和体内证据

• DOI: 10.1016/j.jdermsci.2010.03.008
• 发表时间: 2010
• 期刊: J Dermatol Sci
• 影响因子: 4.6
• 作者: Matsuda N;Teramae H;Futatsugi M;Takano K;Yamamoto S;Tomita K;SuzukiT;Yokoo H;Koike K;Hattori Y;Inui S et al.(Itami S ; 6人中6番)
• 通讯作者: Inui S et al.(Itami S ; 6人中6番)

PPAR binding protein (PBP/Med1) is involved in signaling for quiescence of hair follicle stem cells.

PPAR 结合蛋白 (PBP/Med1) 参与毛囊干细胞静止的信号传导。

• 发表时间: 2009
• 作者: 中島武之;他
• 通讯作者: 他

Disruption of PPAR binding protein in keratinocyte prevents DMBA/TPA-induced carcinogenesis

角质形成细胞中 PPAR 结合蛋白的破坏可预防 DMBA/TPA 诱导的癌变

• 发表时间: 2009
• 作者: Yutaka Kitagawa;Takeshi Nakajima;Fumihito Noguchi;Shigeki Inui;Satoshi Itami
• 通讯作者: Satoshi Itami

Effect of visible lights on dermal fibroblasts

可见光对真皮成纤维细胞的影响

• 发表时间: 2009
• 作者: Tomohiro Fushimi;Takeshi Nakajima;Shigeki Inui;Koh Hosokawa;Satoshi Itami
• 通讯作者: Satoshi Itami

Acceleration of skin wound healing in PPAR binding protein null mouse

PPAR 结合蛋白缺失小鼠皮肤伤口愈合加速

• 发表时间: 2010
• 作者: 野口史人、中島武之;他
• 通讯作者: 他