A research for new cancer vaccine with heat shock protein for esophageal cancer

食管癌热休克蛋白新型癌症疫苗的研究

• 批准号: 21791268
• 负责人: AKUTSU Yasunori
• 金额: $ 2.66万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791268/
• 关键词: 食道癌; ヒートショックプロテイン; gp96; ヒートショックプロティン

We developed a procedure for extraction of tumor-derived HSP-peptide complex from from tumor using mouse squamous cell carcinoma SCCVII. Purification was carried out according to Pramod K. Srivastav's method (METHODS : A Companion to Methods in Enzymology 12, 165.171 (1997)). Then using clinical specimens, we evaluated the expression of gp96 and prognosis of esophageal cancer patients that underwent surgery and analyzed a correlation between gp96 expression and surgical outcome.In surgical specimens, 73% of all cases expressed gp96. A positive rate of lymph node metastases was higher in cases with negative gp96 expression. By univariate analysis, gp96 positivity indicated good survival compared to negative cases (p=0.049). By multivariate analysis, negativity for gp96 was an independent prognostic factor(Hazard ratio : 2.6, p=0.04). The number of metastatic lymph nodes was much higher in gp96 negative cases. These results indicated that gp96 can be a useful biomarker for esophageal cancer. These result has been published in a scientific journal.

我们开发了一种从小鼠鳞状细胞癌SCCVII中提取肿瘤来源的HSP-肽复合物的方法。根据Pramod K进行纯化。Srivastav的方法（METHODS：A Companion to Methods in Enzymology 12，165.171（1997））。然后，我们使用临床标本，评估gp 96的表达和手术治疗的食管癌患者的预后，并分析gp 96表达与手术预后的相关性。gp 96表达阴性者淋巴结转移阳性率较高。通过单变量分析，与阴性病例相比，gp 96阳性表明良好的存活率（p=0.049）。通过多变量分析，gp 96阴性是一个独立的预后因素（风险比：2.6，p=0.04）。gp 96阴性者淋巴结转移明显增多。这些结果表明，gp 96可以作为食管癌的一个有用的生物标志物。这些结果已发表在一份科学杂志上。

项目成果

食道扁平上皮癌におけるリンパ節転移と免疫マーカーとしてのgp96発現の検討

食管鳞癌淋巴结转移及gp96免疫标志物表达检测

• 发表时间: 2011
• 作者: Kondo M;Izumi T;Fujieda N;Kondo A;Morishita T;Matsushita H;Kakimi K;阿久津泰典
• 通讯作者: 阿久津泰典

Correlation Between gp96 Expression and the Surgical Outcome in Patients With Esophageal Squamous Cell Carcinoma

• DOI: 10.1245/s10434-010-1128-7
• 发表时间: 2011-03-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Akutsu, Yasunori;Matsubara, Hisahiro;Yusup, Gulbostan
• 通讯作者: Yusup, Gulbostan

食道癌における免疫学的バイオマーカーとしてのgp96発現の意義

gp96表达作为食管癌免疫生物标志物的意义

• 发表时间: 2011
• 作者: Kondo M;Izumi T;Fujieda N;Kondo A;Morishita T;Matsushita H;Kakimi K;阿久津泰典
• 通讯作者: 阿久津泰典

Antitumor effect for squamous cell carcinoma mediated by heat shock protein gp96

热休克蛋白gp96介导的鳞状细胞癌抗肿瘤作用

• 发表时间: 2010
• 作者: Y. Akutsu;et al
• 通讯作者: et al

COX2 Expression Predicts Resistance to Chemoradiotherapy in Esophageal Squamous Cell Carcinoma

• DOI: 10.1245/s10434-011-1645-z
• 发表时间: 2011-10-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Akutsu, Yasunori;Hanari, Naoyuki;Matsubara, Hisahiro
• 通讯作者: Matsubara, Hisahiro