Approaches to regenerate pancreatic function by converting liver blood flow and transferring specific gene

免疫组织化学研究发现，肝脏中既有抗胰岛素阳性细胞，又有抗胰蛋白酶阳性细胞，阻断门脉血流，但该未成熟细胞团没有任何分泌颗粒。在本研究中，我们试图通过基因治疗将分化促进因子引入该簇，并产生胰腺内分泌和外分泌细胞。在本研究期间，虽然我们试图用噬菌体载体向成团细胞传递分化促进因子，但在肝脏和胆管中都找不到足够数量的表达载体的细胞。我们将在未来继续为它制作载体。

Although we found the both anti-insulin and anti-trypsin positive cells in the liver that interrupted portal blood flow by the immunohistochemical study, that immature cell cluster didn' t have any secretary granules. In this study, we attempted to introduce differentiation enhancing factor to the cluster by gene therapy, and generate pancreatic endocrine and exocrine cells. Although we try to make phage vector to deliver differentiation enhancing factor to the cluster for these research period, we couldn' t find adequate amount vector-transfected cells in both the liver and bile duct. We will continue to make vector for it in the future.