Establishment and characterization of cancer stem cell model using iPS technology

利用iPS技术建立癌症干细胞模型并表征

• 批准号: 21791307
• 负责人: FUKUDA Kazumasa
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791307/
• 关键词: iPS; 人工癌幹細胞; 胃癌; リプログラミング; 癌幹細胞

We demonstrate induction of iCaPS from human gastric cancer (GC) cell lines and primary culture cell derived from the patients with GC introducing Yamanaka four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. These cells, which we designated iCaPS, exhibit the morphology and growth properties of iPS cells. These data suggest that CSCs (cancer stem-like cells) can be directly generated from human GC cell liens by the addition of only a few defined factors. We analysed expression of alkaline phosphatase. These date indicated that iCaPS cells acquired immature status. WST-8 assay showed that iCaPS cells were acqired low sensitivityto fluorouracil, cisplatin and PTX compred with wild type cell. As novel therapeutic approches in GC, the properties of iCaPS should bebe investigaged.

我们证明了来自ES细胞培养条件下的Yamanaka诱导ICAP，并从GC引入Yamanaka的患者OCT3/4，SOX2，C-MYC和KLF4的患者中诱导了原发性培养细胞。这些细胞（我们指定ICAPS）表现出IPS细胞的形态和生长特性。这些数据表明，CSC（癌症样细胞）可以通过仅添加几个定义因子直接从人类GC细胞留置权中产生。我们分析了碱性磷酸酶的表达。这些日期表明ICAPS细胞获得了未成熟状态。 WST-8测定法显示，ICAPS细胞对氟尿嘧啶，顺铂和PTX的敏感性低敏感性，由野生型细胞组成。正如GC中新型的治疗方法一样，应研究ICAP的特性。