Establishment and characterization of cancer stem cell model using iPS technology

在ES细胞培养条件下，我们研究了人胃癌细胞株和山中四因子(Oct3/4，Sox2，c-Myc，KLF4)对人胃癌细胞株和原代培养细胞的诱导作用。这些细胞具有iPS细胞的形态和生长特性，我们将其命名为ICaps。这些数据表明，只需添加几个确定的因子，就可以直接从人的GC细胞留置中产生CSCs(癌症干细胞样细胞)。我们分析了碱性磷酸酶的表达。这些数据表明ICaps细胞获得了未成熟状态。WST-8检测显示ICaps细胞对氟尿嘧啶、顺铂和PTX的敏感性低于野生型细胞。ICap作为GC治疗的新途径，其特性值得进一步研究。

We demonstrate induction of iCaPS from human gastric cancer (GC) cell lines and primary culture cell derived from the patients with GC introducing Yamanaka four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. These cells, which we designated iCaPS, exhibit the morphology and growth properties of iPS cells. These data suggest that CSCs (cancer stem-like cells) can be directly generated from human GC cell liens by the addition of only a few defined factors. We analysed expression of alkaline phosphatase. These date indicated that iCaPS cells acquired immature status. WST-8 assay showed that iCaPS cells were acqired low sensitivityto fluorouracil, cisplatin and PTX compred with wild type cell. As novel therapeutic approches in GC, the properties of iCaPS should bebe investigaged.