Identification and function analysis of cancer stem cell in esophageal cancer

纤维蛋白原是食道癌的预后因素之一。在食管鳞癌细胞系中，整合素β3(CD61/纤维蛋白原受体)阳性细胞系(TE8)在含有生长因子(EGF和bFGF)的无血清培养液中呈球状。这一结果提示CD61阳性细胞具有肿瘤干细胞自我更新的特性。进一步对细胞形态功能进行分析，CD61阳性细胞表明其具有较高的趋化能力。另一方面，通过长期接触抗癌剂(5FU)对食管鳞癌细胞株TE10和TE11进行肿瘤干细胞的纯化。6个月后，两种细胞均获得耐药能力，并检测miRNAs(miR-200家族或miR-let-7家族)的表达变化。

Fibrinogen is one of the prognostic factors in esophageal cancer. In esophageal squamous carcinoma cell lines, integrin beta 3 (CD61/fibrinogen receptor) positive cell line (TE8) showed sphere-formation in serum-free medium containing growth factors (EGF and bFGF). This result suggested that CD61- positive cells have the property of self-renewal of cancer stem cell. Furthermore, the analysis of cell morphology functions, CD61-positive cells indicated the high chemotactic ability. On the other hand, to enrich for cancer stem cells, followed by purification of cancer stem cells by long-term exposure to anti-cancer agents (5FU) in esophageal squamous carcinoma cell lines TE10 and TE11. After six months, both cells have acquired the ability for drug resistance, and the expression variation of miRNAs (miR-200 family or miR-let-7 family) were detected.