Control of intracellular signaling, involved in neuronal differentiation by using protein transduction domain and its application

利用蛋白转导域控制参与神经元分化的细胞内信号传导及其应用

• 批准号: 21791374
• 负责人: KUBO Atsuhiko
• 金额: $ 2.91万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791374/
• 关键词: 神経分化; ペプチド; 皮膚由来幹細胞; パーキンソン病; STAT3; VHL; 幹細胞移植; 膜貫通タンパク; SOCS

As central nervous system is not regenerated naturally, reproduce of the damaged brain/neuron is expected by transplant of neuronal stem cells. Topics clarified in this study are 1 ; neuronal differentiation potential of skin-derived precursor cells 2 ; control of an intra-cellular factor involved in neuronal differentiation by using peptide. We identified the skin-derived precursor cells had neuronal differentiation ability and the advantage that there was no necessary of gene manipulation for the cells from isolation to transplant procedure. I designed the peptide, there was little cytotoxicity, which introduced to control intracellular signaling enrolled of neuronal differentiation inducing factor. It was thought that the utilization of the skin-derive precursor cells were useful as exclusive law of the regenerative medicine at this time

由于中枢神经系统不能自然再生，因此通过移植神经元干细胞来再生受损的大脑/神经元是有希望的。在这项研究中澄清的主题是1 ;神经元分化的皮肤来源的前体细胞的潜力2 ;通过使用肽参与神经元分化的细胞内因子的控制。我们发现皮肤来源的前体细胞具有向神经元分化的能力，并且从分离到移植过程中不需要基因操作。设计了一种细胞毒性很小的肽段，引入调控细胞内信号转导的神经元分化诱导因子。人们认为，皮肤来源的前体细胞的利用是有用的，这是当时再生医学的唯一法则

项目成果

多能性幹細胞の神経分化を誘導する機能性ペプチドを用いた神経再生医療の開発

使用诱导多能干细胞神经分化的功能性肽开发神经再生医学

• 发表时间: 2011
• 作者: 菅野洋;久保篤彦;東田哲博;伊藤典彦
• 通讯作者: 伊藤典彦

Skin-derived precursors differentiating into dopaminergic neuronal cells in the brains of Parkinson disease model rats.

帕金森病模型大鼠大脑中皮肤来源的前体细胞分化为多巴胺能神经元细胞。

• 发表时间: 2010
• 期刊: Journal of Neurosurgery
• 影响因子: 4.1
• 作者: Higashida T;Jitsuki S;Kubo A;et al.
• 通讯作者: et al.

Efficient Generation of Dopamine Neuron-Like Cells From Skin-Derived Precursors With a Synthetic Peptide Derived From von Hippel-Lindau Protein

• DOI: 10.1089/scd.2008.0379
• 发表时间: 2009-12-01
• 期刊: STEM CELLS AND DEVELOPMENT
• 影响因子: 4
• 作者: Kubo, Atsuhiko;Yoshida, Tetsuhiko;Kanno, Hiroshi
• 通讯作者: Kanno, Hiroshi

Engrafted VHL peptide-delivered bone marrow stromal cells promote spinal cord repair in rats

• DOI: 10.1097/wnr.0b013e328336ee9a
• 发表时间: 2010-03-10
• 期刊: NEUROREPORT
• 影响因子: 1.7
• 作者: Yamazaki, Yoshiyuki;Kanno, Hiroshi;Saito, Tomoyuki
• 通讯作者: Saito, Tomoyuki

Peptide Treatment Determinate the Neural Fate of Skin-derived Precursors and Its Application into Pakinson's Disease

肽治疗确定皮肤源性前体的神经命运及其在帕金森病中的应用

• 发表时间: 2009
• 作者: Kubo A;Yokoyama T;Mimura T;Kanno H.
• 通讯作者: Kanno H.