Inhibition of tumor growth and sensitization to chemotherapy by RNA interference targeting Aurora-A in the human bladder cancer KoTCC1 model.

在人膀胱癌 KoTCC1 模型中，通过针对 Aurora-A 的 RNA 干扰抑制肿瘤生长和对化疗敏感。

• 批准号: 21791502
• 负责人: SAKAI Iori
• 金额: $ 2.08万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791502/
• 关键词: Aurora-A; siRNA; 膀胱癌; 抗がん剤感受性; shRNA; Aurora kinase

OBJECTIVES : To investigate the inhibitory effects of Aurora-A expression in bladder cancer cells on their growth and chemosensitivity. METHODS : Aurora-A expression in several human bladder cancer cell lines were evaluated by real time RT-PCR. We then established KoTCC1 cells in which the expression vector containing short-hairpin RNA (shRNA) targeting Aurora-A was introduced (KoTCC1/Aurora). The growth and the sensitivity to several therapeutic agents in KoTCC1/Aurora were compared with those in KoTCC1 transfected with control vector alone (KoTCC1/Co). Expression levels of both Aurora-A mRNA and protein in KoTCC1/Aurora were 20% of those in KoTCC1/Co. In vitro growth of KoTCC1/ Aurora was significantly worse than that of KoTCC1/Co. The 50% inhibitory concentration of docetaxel and sunitninb in KoTCC1/Aurora decreased compared with that in KoTCC1/Co. CONCLUSIONS : The suppression of Aurora-A using shRNA could be a useful therapeutic strategy against bladder cancer, through growth inhibition as well as enhanced chemosensitivity.

目的：探讨Aurora-A在膀胱癌细胞中的表达对其生长和化疗敏感性的抑制作用。方法：采用实时荧光定量RT-PCR方法检测Aurora-A在人膀胱癌细胞系中的表达。在此基础上，我们建立了含有针对Aurora-A的短发夹状RNA(ShRNA)的表达载体(KoTCC1/Aurora)的KoTCC1细胞。比较KoTCC1/Aurora细胞与单纯转导对照载体的KoTCC1细胞(KoTCC1/Co)的生长及对几种药物的敏感性。KoTCC1/Aurora细胞中Aurora-A基因和蛋白的表达水平均为KoTCC1/Co的20%，KoTCC1/Aurora细胞的体外生长明显低于KoTCC1/Co，多西紫杉醇和舒尼替尼的50%抑制浓度低于KoTCC1/Co。结论：shRNA抑制Aurora-A基因可通过抑制细胞生长和增强化疗敏感性而成为治疗膀胱癌的有效策略。

项目成果

Expression profile of E-cadherin and N-cadherin in non-muscle-invasive bladder cancer as a novel predictor of intravesical recurrence following transurethral resection

• DOI: 10.1016/j.urolonc.2010.01.005
• 发表时间: 2012-03-01
• 期刊: UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
• 影响因子: 2.7
• 作者: Muramaki, Mototsugu;Miyake, Hideaki;Fujisawa, Masato
• 通讯作者: Fujisawa, Masato

Inhibition of tumor growth and sensitization to chemotherapy by RNA interference targeting interleukin-6 in the androgen-independent human prostate cancer PC3 model.

在不依赖雄激素的人前列腺癌 PC3 模型中，通过靶向白细胞介素 6 的 RNA 干扰抑制肿瘤生长和对化疗的敏感性。

• 发表时间: 2011
• 期刊: Cancer Sci. 102(4)
• 作者: Sakai I;Miyake H;Terakawa T;Fujisawa M.
• 通讯作者: Fujisawa M.

Inhibition of tumor growth and sensitization to chemotherapy by RNA interference targeting Aurora-A in the human bladder cancer KoTCC1 model.

在人膀胱癌 KoTCC1 模型中，通过针对 Aurora-A 的 RNA 干扰抑制肿瘤生长和对化疗敏感。

• 期刊: BJU international
• 影响因子: 4.5
• 作者: Sakai I;Miyake H;Harada K;Hara I;Inoue TA;Fujisawa M.;Iori Sakai
• 通讯作者: Iori Sakai

Assessment of long-term quality of life in patients with orthotopic neobladder followed for more than 5 years

• DOI: 10.1007/s11255-010-9851-3
• 发表时间: 2011-09-01
• 期刊: INTERNATIONAL UROLOGY AND NEPHROLOGY
• 影响因子: 2
• 作者: Takenaka, Atsushi;Hara, Isao;Fujisawa, Masato
• 通讯作者: Fujisawa, Masato

Analysis of factors predicting intravesical recurrence of superficial transitional cell carcinoma of the bladder without concomitant carcinoma in situ

• DOI: 10.1111/j.1442-2042.2006.01562.x
• 发表时间: 2006-11-01
• 期刊: INTERNATIONAL JOURNAL OF UROLOGY
• 影响因子: 2.6
• 作者: Sakai, Iori;Miyake, Hideaki;Fujisawa, Masato
• 通讯作者: Fujisawa, Masato