Roles of branched-type disialyl-glycolipid antigen in renal cell cancer

有报道称，新抗原GalNAc-DSLc4(GalNAc-DSLc4)与肾癌的恶性特征有关。利用TUHR14TKB细胞获得稳定表达GalNAc-T2cDSLc4的TUHR14TKB细胞株，并对其表型进行分析。到目前为止，通过实时细胞电子传感(RT-CES)的动态监测，可以观察到不同细胞外基质的转染体与层粘连蛋白涂层平板的特异性粘附性。为了解释GalNAc-DSLc4转染体在层粘连蛋白涂层平板上的特殊黏附，我们研究了各种黏附分子，如已知的与层粘连蛋白相互作用的整合素。最近，我们利用激光共聚焦显微镜的免疫荧光技术，揭示了整合素β-1和GalNAc-DSLC4抗原在细胞表面的定位。

It has been reported that novel antigen GalNAc-disialyl Lc4 (GalNAc-DSLc4) is associated with malignant features of renal cancers. The stable transfectant of GalNAc-T2 cDNA using TUHR14TKB cells, resulting in the high expression of GalNAc-DSLc4, was established to analyze their phenotypes. To date, specific adhesion of transfectants onto laminin-coating plates among those with various extracellular matrices was observed by the dynamic monitoring using real-time cell electronic sensing (RT-CES). To explain the specific adhesion of GalNAc-DSLc4 transfectants on the laminin-coating plate, we investigated various adhesion molecules such as integrins known to interact with laminin. And recently, we revealed the localization of integrin β1 and GalNAc-DSLc4 antigen on the cell surface by immunofluorescence assay with confocal laser microscopy.