Roles of branched-type disialyl-glycolipid antigen in renal cell cancer

支链型二唾液酸糖脂抗原在肾细胞癌中的作用

• 批准号: 21791534
• 负责人: TSUCHIDA Akiko
• 金额: $ 2.75万
• 依托单位: The Noguchi Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791534/
• 关键词: 腎癌; 糖脂質; 糖転移酵素; ジシアリル糖鎖; 腫瘍マーカー

It has been reported that novel antigen GalNAc-disialyl Lc4 (GalNAc-DSLc4) is associated with malignant features of renal cancers. The stable transfectant of GalNAc-T2 cDNA using TUHR14TKB cells, resulting in the high expression of GalNAc-DSLc4, was established to analyze their phenotypes. To date, specific adhesion of transfectants onto laminin-coating plates among those with various extracellular matrices was observed by the dynamic monitoring using real-time cell electronic sensing (RT-CES). To explain the specific adhesion of GalNAc-DSLc4 transfectants on the laminin-coating plate, we investigated various adhesion molecules such as integrins known to interact with laminin. And recently, we revealed the localization of integrin β1 and GalNAc-DSLc4 antigen on the cell surface by immunofluorescence assay with confocal laser microscopy.

据报道，新抗原GalNAc-二唾液酸Lc 4（GalNAc-DSLc 4）与肾癌的恶性特征相关。利用TUHR 14 TKB细胞建立GalNAc-T2 cDNA的稳定转染子，导致GalNAc-DSLc 4的高表达，以分析其表型。迄今为止，通过使用实时细胞电子传感（RT-CES）的动态监测，观察到转染子在具有各种细胞外基质的层粘连蛋白包被板上的特异性粘附。为了解释GalNAc-DSLc 4转染子在层粘连蛋白包被板上的特异性粘附，我们研究了各种粘附分子，例如已知与层粘连蛋白相互作用的整合素。最近，我们通过激光共聚焦显微镜的免疫荧光分析揭示了整合素β1和GalNAc-DSLc 4抗原在细胞表面的定位。