Functional analysis of cochlear outer hair cells in a dominant-negative connexin26 mutant mouse.

显性失活 connexin26 突变小鼠耳蜗外毛细胞的功能分析。

• 批准号: 21791648
• 负责人: NARUI Yuya
• 金额: $ 2.66万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791648/
• 关键词: gjb2遺伝子; 優性阻害効果; マウス変異体; 歪成分耳音響放射; 外有毛細胞; gib2遺伝子; 内耳; GJB2; ギャップジャンクション

Distortion product otoacoustic emissions (DPOAEs) have been used to examine the development of hearing in the rat and gerbil. However, no reports of DPOAE measurement from the onset of hearing in mice are available. Commercially-available components were assembled and adapted to provide a suitable probe microphone and sound delivery system for measuring DPOAE in developing C57BL/6J mice. Furthermore, DPOAE data were compared with the findings of the auditory brainstem response (ABR). DPOAEs were obtained at 8 kHz from 11 days after birth, 20 kHz from 12 days, and 30 kHz from 13 days. Adult-like patterns of DPOAE were obtained 21 days at 8 and 20 kHz, and 28 days at 30 kHz. On the other hand, the ABR thresholds at 12 to 36 kHz appeared between 11 and 12 days and were saturated at 14 days. Based on these data, the onset of measureable DPOAEs in the mouse were earlier than in the rat and gerbil. The maturation of DPOAE in the mouse begins at a lower frequency in the high frequency range. In addition, the ABR threshold reached maturation earlier than DPOAE.

畸变产物耳声发射（dpoae）被用来研究大鼠和沙鼠的听力发育。然而，没有关于小鼠听力开始时DPOAE测量的报道。组装和改装了市售元件，为开发中的C57BL/6J小鼠的DPOAE测量提供了合适的探针麦克风和声音传递系统。此外，将DPOAE数据与听觉脑干反应（ABR）结果进行比较。出生后11天在8 kHz， 12天在20 kHz， 13天在30 kHz时获得dpoae。在8、20 kHz和30 kHz条件下，分别在21天和28天获得类似成人的DPOAE模式。另一方面，12 ~ 36 kHz的ABR阈值在11 ~ 12天出现，在14天达到饱和。根据这些数据，小鼠可测量的dpoae的发病时间比大鼠和沙鼠早。小鼠体内DPOAE的成熟开始于高频范围内较低的频率。此外，ABR阈值比DPOAE更早达到成熟。